核农学报2025，Vol.39Issue(11)：2374-2387,14.DOI:10.11869/j.issn.1000-8551.2025.11.2374
毛竹CLCs基因家族鉴定及高盐与干旱胁迫下的表达模式分析
Identification and Expression Analysis of CLC Genes in Response to Hypersaline Stress and Drought Stress in Moso Bamboo(Phyllostachys edulis)
摘要
Abstract
Chloride Channel(CLC)protein family represents an important group of channels and transport proteins that mediate the transmembrane anion transport,with particular specificity for chloride ions(Cl-).Extensive studies have demonstrated the essential role of CLCs in plant responses to various abiotic stresses,notably high salinity and drought.To explore the function of the CLC gene family in Moso bamboo(Phyllostachys edulis),we identified and analyzed the members of the CLC gene family in Moso bamboo through bioinformatics analysis,and examined the expression of CLC genes under hypersaline and drought stress using RNA-Seq and qRT-PCR technologies.The results indicate that 18 CLC genes were identified in the Moso genome.These genes encode proteins ranging from 455 to 1 440 amino acids(aa)in length,with corresponding molecular weights of 49 358.04 to 157 276.61 Daltons(Da)and theoretical isoelectric points(pI)between 6.40 and 8.85.Phylogenetic analysis classified the 18 PeCLCs into 2 subclasses and 6 subfamilies,with genes within the same subfamily sharing conserved gene structures.Furthermore,collinearity analysis revealed 9 collinear gene pairs within the CLC gene family in Moso bamboo,all exhibiting Ka/Ks less than 1.Promoter cis-element analysis indicated that the promoter sequences of PeCLCs harbor diverse stress-and hormone-responsive regulative elements.Transcriptome profiling revealed tissue-and developmental stage-specific expression patterns of PeCLC genes,with pronounced enrichment in the rhizome system and bamboo shoots across multiple developmental stages.This indicates that PeCLCs play an important role in Cl-homeostasis maintenance in the rhizome system and during the rapid growth of bamboo shoots.qRT-PCR results demonstrated that the majority of PeCLCs were induced under high salinity and drought stress conditions,supporting their involvement in response to these abiotic stresses.The results of this study establish a foundational framework and provide genetic resources for further functional studies of CLCs in Moso bamboo.关键词
毛竹/CLC基因家族/生物信息学/高盐胁迫/干旱胁迫Key words
Phyllostachys edulis/CLC gene family/bioinformatics/hypersaline stress/drought stress引用本文复制引用
陈诗蕾,郭琳,鲁海雯,叶晓莉,林新春..毛竹CLCs基因家族鉴定及高盐与干旱胁迫下的表达模式分析[J].核农学报,2025,39(11):2374-2387,14.基金项目
"十四五"国家重点研发计划(2021YFD2200503-3) （2021YFD2200503-3）
