核农学报2025，Vol.39Issue(11)：2398-2406,9.DOI:10.11869/j.issn.1000-8551.2025.11.2398
脂联素及其受体和乳酸转运相关蛋白在不同年龄牦牛睾丸的差异表达分析
Differential Expression of Adiponectin,Its Receptor and Monocarboxylate Transporter in Testis of Yaks at Different Ages
摘要
Abstract
Adiponectin plays a crucial role in regulating key metabolic processes such as energy balance,lipid metabolism,and glucose metabolism.To explore the expression and distribution of adiponectin,its receptors,and lactate transport-related proteins in yak testis across lactation,juvenile,adult,and aged stages,and their effects on testicular development,techniques such as quantitative real-time PCR(qRT-PCR),Western blotting,and immunohistochemical staining were employed.The results of qRT-PCR showed that the transcriptional level of APN mRNA gradually increased with the age of yaks.APNR1 mRNA peaked in the aged yaks(P<0.01).APNR2 mRNA showed significantly higher expression in juvenile and aged than in lactation and adult yaks(P<0.01).MCT1 mRNA transcription was significantly higher in adult and aged than in lactation and juvenile yaks(P<0.01),while MCT2 mRNA levels was significantly higher in juvenile and aged than in lactation and adult yaks(P<0.01).The results of western blotting demonstrated that the protein expression level of APN was significantly higher in adult and aged yaks than in the lactation and juvenile yaks(P<0.01).The protein level of APNR1 in aged yaks was the highest(P<0.01).APNR2 was significantly higher in juvenile and aged than in lactation and adult yaks(P<0.01).The protein expression level of MCT1 gradually increased with the age of yaks,and the protein expression level of MCT2 was significantly higher in juvenile and aged than in lactation and adult yaks(P<0.01).The expression trends of adiponectin,its receptor proteins,and lactate transport-related proteins were basically consistent with those of their corresponding mRNAs.I Immunohistochemical analysis showed that APN,APNR1,APNR2,MCT1,and MCT2 were predominantly distributed in Sertoli cells,Leydig cells,and spermatogenic cells in the testis.Compared with MCT2,MCT1 showed a strong positive expression.The results indicated that the expression levels of APNR1 and MCT1 proteins in yak testis were significantly higher after sexual maturity than those before sexual maturity,suggesting that both two proteins play important roles in energy regulation and lactate transport within yak testis after sexual maturity.This study provides experimental data for exploring reproductive regulation mechanism of yaks in plateau environment.关键词
牦牛/脂联素/乳酸转运蛋白Key words
yak/adiponectin/monocarboxylate transporter引用本文复制引用
张虔,马睿,崔燕,余四九..脂联素及其受体和乳酸转运相关蛋白在不同年龄牦牛睾丸的差异表达分析[J].核农学报,2025,39(11):2398-2406,9.基金项目
国家自然科学基金项目(32473110) （32473110）
