内蒙古民族大学学报（自然科学版）2025，Vol.40Issue(5)：20-29,10.DOI:10.14045/j.cnki.15-1220.2025.05.004
钝顶螺旋藻藻蓝蛋白抗氧化酶解产物的制备及活性研究
Study on Preparation and Activity of Antioxidant Enzymatic Hydrolysates of Phycocyanin from Spirulina platensis
摘要
Abstract
To obtain the highly efficient antioxidant enzymatic hydrolysate of phycocyanin from Spirulina platen-sis,phycocyanin was used as the raw material.The DPPH·scavenging rate was taken as the index.Based on the screening of the enzymes for enzymatic hydrolysis,the single factor experiment combined with the Box-Behnken central composite response surface method was used to establish the optimal enzymatic hydrolysis process.The enzy-matic hydrolysate prepared under the optimal process was evaluated for antioxidant activity and then preliminarily purified.The results showed that the optimal enzyme for enzymatic hydrolysis was alkaline protease.The optimal en-zymatic hydrolysis process parameters for the highly efficient antioxidant enzymatic hydrolysate were as follows:en-zyme addition amount of 10 000 U/g,enzymatic hydrolysis time of 56 min,enzymatic hydrolysis temperature of 51.5℃,and enzymatic hydrolysis pH of 10.2.Under this optimal process,when the mass concentration of the enzy-matic hydrolysate was 20.00 mg/mL,the DPPH·scavenging rate was 30.33%;when the mass concentration was 7.50 mg/mL,the·OH scavenging rate was 90.86%;when the mass concentration was 0.12 mg/mL,the ABTS+·scav-enging rate was 83.49%;when the mass concentration was 10.91 mg/mL,the·O2ˉ scavenging rate was 55.60%.Af-ter purification with 80%ethanol,the DPPH·and ABTS+·scavenging rates of the purified enzymatic hydrolysate in-creased by 2.3 times and 3.8 times respectively compared to those before purification.The difference in scavenging rates against two types of radicals demonstrated statistical significance(P＜0.05)before and after purification of the enzymatic hydrolysates.The quadratic model equation developed in this study demonstrated high reliability(pre-dicted R2=0.905)and can be used to predict the optimal enzymolysis process for obtaining highly efficient antioxi-dant enzymatic hydrolysates,providing a data basis for the preparation of antioxidant peptides from S.platensis by enzymatic hydrolysis and the development of anti-aging special dietary foods.关键词
钝顶螺旋藻/藻蓝蛋白/抗氧化活性/酶解工艺Key words
Spirulina platensis/phycocyanin/antioxidant activity/enzymatic hydrolysis process分类
医药卫生引用本文复制引用
陈朝阳,曾欣怡,吴婷,纪思莉,宋国强,王芳..钝顶螺旋藻藻蓝蛋白抗氧化酶解产物的制备及活性研究[J].内蒙古民族大学学报（自然科学版）,2025,40(5):20-29,10.基金项目
福建省自然科学基金面上项目(2022J011109,2023J01904) （2022J011109,2023J01904）
泉州市科技计划项目海洋领域项目(2022N040) （2022N040）
2023年度福厦泉国家自主创新示范区泉州片区协同创新平台项目(2023FX0001) （2023FX0001）
2022年福建省大学生创新创业训练计划项目(S202210399046) （S202210399046）
