农业生物技术学报2025，Vol.33Issue(10)：2112-2123,12.DOI:10.3969/j.issn.1674-7968.2025.10.002
HvSWEET4基因启动子克隆及其活性分析
Cloning and Activity Analysis of the HvSWEET4 Gene Promoter
摘要
Abstract
HvSWEET4,a member of the sugars will eventually be exported transporters(SWEET)sugar transporter family in hulless barley(Hordeum vulgare var.nudum),is involved in hexose transport regulation during grain development.To elucidate the transcriptional regulatory mechanism of HvSWEET4,approximately 2 000 bp of promoter sequences in upstream of the coding region were systematically analyzed from 2 hulless barley cultivars with significantly different grain sizes:'Beiqing 3'(large grain)and'3917'(small grain).Sequence comparison revealed that the HvSWEET4 promoters from these 2 cultivars were highly conserved,containing only 10 SNPs and 5 InDels,which accounted for merely 0.75％of the total nucleotides.Cis-element analysis indicated that the HvSWEET4 promoter contained regulatory elements associated with stress responses,sugar and starch metabolism and developmental regulation of pollen,endosperm and aleurone layers.Promoter activity assays identified the core functional region at-310～-212 bp.Further analyses suggested that differences in promoter activity could be attributed to suppressor effects within the region from-1 999～-310 bp,as well as SNP variations in the region from-212～-1 bp,potentially altering the types and sites of transcription factor binding.These findings provide essential theoretical foundations for further dissecting the molecular mechanisms underlying HvSWEET4 role in grain size regulation and highlight its potential applications in crop breeding.关键词
大麦/HvSWEET4/启动子活性Key words
Barley/H.vulgare var.nudum sugars will eventually be exported transporters(HvSWEET4)/Promoter activity分类
农业科技引用本文复制引用
尤恩,王一航,沈裕虎,徐金青,王蕾,王寒冬,陈同睿,唐有林,邓超,边海燕,卢露..HvSWEET4基因启动子克隆及其活性分析[J].农业生物技术学报,2025,33(10):2112-2123,12.基金项目
国家自然科学基金联合基金区域创新发展联合基金(U22A20453) （U22A20453）
国家自然科学基金面上项目(32171958) （32171958）
青海省重点研发与转化计划项目-科技成果转化专项项目(2023-NK-101) （2023-NK-101）
国家重点研发计划部省联动项目(2022YFD2301301) （2022YFD2301301）
西藏自治区重点研发与转化项目(XZ202501ZY0029) （XZ202501ZY0029）
中国科学院青海省人民政府三江源国家公园联合专项(LHZX-2022-03) （LHZX-2022-03）
