农业生物技术学报2025，Vol.33Issue(10)：2124-2136,13.DOI:10.3969/j.issn.1674-7968.2025.10.003
海岛棉组蛋白去甲基化酶基因GbJMJ25应答干旱胁迫的功能初步鉴定
Preliminary Functional Characterization of the Histone Demethylase Gene GbJMJ25 from Sea Island Cotton(Gossypium barbadense)in Response to Drought Stress
摘要
Abstract
Sea Island cotton(Gossypium barbadense)is renowned for its superior fiber quality and high economic value.However,drought stress significantly impacts its yield and fiber quality.Identifying drought stress-related genes as candidate genes for molecular design breeding is one of the key strategies to enhance drought resistance in Sea Island cotton.In this study,the gene GbJMJ25(JMJ:Jumonji),encoding a histone demethylase in Sea Island cotton,was cloned using RT-PCR.Bioinformatics analysis,expression profiling,and subcellular localization of GbJMJ25 were conducted,and its potential role in drought stress response was preliminarily validated using virus-induced gene silencing(VIGS)technology.Bioinformatics analysis revealed that GbJMJ25 encoded a protein of 1 055 amino acids,containing 2 conserved domains,RING and JmjC.The promoter region of GbJMJ25 contained multiple cis-acting elements related to light response,growth and development,and stress response.Expression pattern analysis demonstrated that GbJMJ25 was responsive to abscisic acid(ABA)and polyethylene glycol(PEG)treatments.Subcellular localization analysis indicated that GbJMJ25 protein was localized in the nucleus.Functional validation via VIGS technology showed that under normal conditions,GbJMJ25-silenced cotton plants exhibited no significant phenotypic differences compared with the control(pTRV2)plants.However,under drought stress,GbJMJ25-silenced plants displayed enhanced tolerance,with a higher survival rate than the control,suggesting that GbJMJ25 might play a role in cotton's drought stress response.The results of this study provide candidate genes for improving drought resistance in Sea Island cotton through molecular design breeding.关键词
海岛棉/GbJMJ25/干旱胁迫/表达模式/病毒诱导基因沉默(VIGS)Key words
Sea Island cotton/GbJMJ25/Drought stress/Expression patterns/Virus-induced gene silencing(VIGS)分类
农业科技引用本文复制引用
王晓云,李悦,麦伍兰·图尔荪,米合日巴·阿力木,孙兴菊,张霞..海岛棉组蛋白去甲基化酶基因GbJMJ25应答干旱胁迫的功能初步鉴定[J].农业生物技术学报,2025,33(10):2124-2136,13.基金项目
国家自然科学基金(2520JZRGG) （2520JZRGG）
