农业生物技术学报2025，Vol.33Issue(10)：2137-2148,12.DOI:10.3969/j.issn.1674-7968.2025.10.004
光皮桦BlKNOX7基因的克隆与功能研究
Cloning and Functional Study of BlKNOX7 Gene in Betula luminifera
摘要
Abstract
The KNOX(KNOTTED 1-like homeobox gene)family serves as a core regulatory factor family for plant growth and development.Abnormal functions of this family can result in phenotypes such as organ malformations in plants.Therefore,analyzing its regulatory mechanism is of great significance for basic plant research and crop genetic improvement.In this study,the BlKNOX7 gene from Betula luminifera was cloned and its sequence characteristics were analyzed using bioinformatics methods.The expression patterns of the gene in various tissues and during tension wood formation were examined through qRT-PCR.Additionally,the phenotypic characteristics of transgenic Arabidopsis thaliana overexpressing BlKNOX7 were evaluated.The results showed that the BlKNOX7 gene(GenBank No.PV577094)consisted of 5 exons and 4 introns,with a cDNA sequence length of 1 095 bp and an ORF of 891 bp,encoding 296 amino acids.Evolutionary tree analysis revealed that B1KNOX7 clustered with the reported secondary wall development-associated transcription factor,Populus trichocarpa KNAT7.The expression of the BlKNOX7 gene in stems increased with the progress of lignification and reached the highest level in leaves.During the induction of tension wood,the expression level of BlKNOX7 in tension wood was significantly lower than that in the control.Overexpression of BlKNOX7 in Arabidopsis thaliana resulted in wavy curled plant leaves;Cross-sections of inflorescence stems stained with toluidine blue showed lighter coloration and a smaller range of coloration than the wild type,suggesting less lignin deposition.qRT-PCR results showed that BlKNOX7 overexpression significantly suppressed the expression of the Arabidopsis secondary wall synthesis pathway key enzyme genes phenylalanine ammonialyase(AtPAL)、4-coumarate-COA ligase(At4CL)、irregular xylem(AtIRX)and cellulose synthase(AtCesA).This study provides a theoretical basis for further investigation of the molecular mechanisms of secondary wall development in Betula luminifera.关键词
光皮桦/BlKNOX7基因/次生壁发育/表达模式Key words
Betula luminifera/BlKNOX7 gene/Secondary cell wall development/Expression pattern分类
农业科技引用本文复制引用
于清,胡晓晴,庄和必,刘雪羽,刘明彤,黄华宏..光皮桦BlKNOX7基因的克隆与功能研究[J].农业生物技术学报,2025,33(10):2137-2148,12.基金项目
浙江省农业新品种选育重大科技专项(2021C02070-1) （2021C02070-1）
