农业生物技术学报2025，Vol.33Issue(10)：2174-2185,12.DOI:10.3969/j.issn.1674-7968.2025.10.007
开食料中添加纤维素酶对性成熟前湖羊睾丸发育与精子发生的影响
Effects of Adding Cellulase to Starter Feed on Testicular Development and Spermatogenesis in Pre-sexually Mature Hu Sheep(Ovis aries)
摘要
Abstract
The testis is an important reproductive organ in male animals,capable of secreting hormones and producing sperm.Cellulase is a key factor in breaking down plant cell walls and enhancing the nutrient absorption ability of ruminants.The level of energy supply directly affects testicular development,so it is hypothesized that the addition of cellulase may have an effect on the testicular development of Hu sheep(Ovis aries)lambs.In order to study the effect of adding cellulase to starter feed on testicular development and spermatogenesis of male lambs of Hu sheep before sexual maturity,24 10-day-old male Hu sheep lambs of similar body weight were selected.They were randomly divided into 2 groups of 12 lambs each.0.1％cellulase was added to the feed of the experimental group,and 6 lambs were slaughtered at random in each group at 60 and 120 d of age.Testicular tissues were collected for gross dissection,histological observation,hormone determination,and the expression of hormone secretion-related genes and reproductive marker genes detection.The results showed that the testicular indices of Hu sheep lambs at 60 and 120 d of age were extremely significantly higher than those of the control group after the addition of cellulase to the starter diet(P＜0.01),and the diameters of the seminiferous tubules increased extremely significantly(P＜0.01),and the lumens of the fine tubules appeared by the 120 d of age,with a lumen area that was significantly larger than the lumen area of the control group(P＜0.05).Spermatogenic epithelial thickness was extremely significantly(P＜0.01)higher than that of the control group at 60 d of age,and the difference was not significant by 120 d of age.Androgen levels in the testes of lambs in the 60 and 120 d of age test groups were extremely significantly higher than those in the control group(P＜0.001),and the mRNA expression levels of androgen synthesis-related genes(steroidogenic acute regulatory protein(StAR)and cytochrome P450 family 17 subfamily A member 1(CYP17A1))showed the same trend;and the mRNA expression levels of the germ cell marker genes of testis of lambs at 60 and 120 d of age DEAD-box helicase 4(DDX4)and the gene of deleted in azoospermia like(DAZL)were the same as that of the other genes.Both genes exhibited significantly higher protein and mRNA expression levels compared to the control group(P＜0.05).The above results showed that the addition of cellulase to the starter diet could promote the testicular development of male lambs of Hu sheep before sexual maturity,promote the development of the varicose fine tube,increase the level of androgen secretion,and then be able to improve the ability of spermatogenesis.This study provides a theoretical basis for the application of cellulase in open diets for young ruminants.关键词
纤维素酶/湖羊/睾丸/雄激素/生殖细胞标记基因Key words
Cellulase/Hu sheep/Testis/Androgen/Germ cell marker genes分类
农业科技引用本文复制引用
郭雅静,任春燕,任思语,崔晶晶,赵芝慧,李明娜,王继卿,郝志云..开食料中添加纤维素酶对性成熟前湖羊睾丸发育与精子发生的影响[J].农业生物技术学报,2025,33(10):2174-2185,12.基金项目
国家自然科学基金(32160800 （）
31960664) （）
甘肃省高校教师创新基金(2025A-083) （2025A-083）
兰州市青年科技人才创新项目(2023-QN-187) （2023-QN-187）
甘肃农业大学科技创新基金-青年导师扶持基金(GAU-QDFC-2024-04) （GAU-QDFC-2024-04）
甘肃农业大学学科团队项目(GAU-XKTD-2022-21) （GAU-XKTD-2022-21）
