农业生物技术学报2025，Vol.33Issue(10)：2224-2232,9.DOI:10.3969/j.issn.1674-7968.2025.10.011
利用GFP标记菌株研究禾谷丝核菌在小麦中的定殖
Study on the Colonization of Rhizoctonia cerealis in Wheat(Triticum aestvum)Using GFP Labeled Strains
摘要
Abstract
As a common soil-borne fungal disease,wheat sharp eyespot has emerged as a crucial factor constraining wheat(Triticum aestvum)yield.In this study,a polyethylene glycol(PEG)-mediated genetic transformation system was employed to transform green fluorescent protein(GFP)into Rhizoctonia cerealis and obtained a transformant that was genetically stable;The GFP-labeled R.cerealis was inoculated to wheat seedlings,and the colonization pattern of R.cerealis in wheat was investigated by section observation.It was found that positive transformants were attained by PEG-mediated transformation onto protoplasts and screened in PDA medium containing 100 mg/mL hygromycin.PCR detection and fluorescence observation indicated that the plasmid carrying GFP had been successfully transfected into the R.cerealis.After successive passaging culture,the positive transformants were able to express stably and their pathogenicity was consistent with that of the wild type.On the fourth day after inoculating the transformants to wheat,distinct fluorescence could be observed in the xylem of the wheat root,and on the seventh day,a fluorescence phenomenon emerged in the phloem of the wheat coleoptile.This experiment successfully obtained R.cerealis with GFP labeling which could be stably inherited and preliminarily elucidated the colonization pattern of R.cerealis in wheat,it provide theoretical basis for further in-depth research on the pathogenic mechanism of R.cerealis and the use of microbial fertilizers to control wheat sharp eyespot.关键词
禾谷丝核菌/GFP/遗传转化/荧光标记/定殖模式Key words
Rhizoctonia cereadis/GFP/Genetic transformation/Fluorescence labeling/Colonization pattern分类
农业科技引用本文复制引用
张敬敬,付佳佳,刘娜,韩胜芳,侯春燕,王冬梅..利用GFP标记菌株研究禾谷丝核菌在小麦中的定殖[J].农业生物技术学报,2025,33(10):2224-2232,9.基金项目
中央引导地方科技发展资金项目(科技创新基地项目)(2020-2022 （科技创新基地项目）
206Z6502G) （）
