农业科学研究2025，Vol.46Issue(3)：17-24,8.
西瓜枯萎病拮抗菌假单胞菌的筛选、鉴定及其防治效果
Screening,Identification and Control Effect of Antagonistic Pseudomonas Against Watermelon Fusarium Wilt
摘要
Abstract
Watermelon Fusarium wilt,caused by Fusarium oxysporum,leads to significant yield loss in watermelon crops.This study aimed to isolate Pseudomonas strains with strong antagonistic effects against watermelon fusarium wilt and to evaluate their plant growth-promoting effects.The antibacterial efficacy of various strains against a range of pathogens was tested and their mor-phological,physiological,biochemical,genetic characteristics and ability to promote plant growth were assessed.A total of 525 bacterial strains were screened,identifying three biocontrol strains——Pseudomonas aeruginosa P2-1,P3-1 and P3-4——that sig-nificantly inhibited the growth of Fusarium oxysporum f.sp.citrulli.These strains demonstrated the ability to fix nitrogen,solubi-lize organic and inorganic phosphorus,produce siderophores,and exhibit protease and lipase enzyme activities,which contributed to increased plant height,stem diameter,chlorophyll content,and both fresh and dry weights of aboveground and underground parts of watermelon seedlings.Additionally,the biocontrol efficacy of these strains against watermelon fusarium wilt in pot experi-ments ranged from 62.29％～63.93％.Membership function analysis ranked their disease resistance and growth promotion effects in the following order:P3-4,P3-1,P2-1,CK.Strain P3-4 shows potential as a biological control agent for managing watermelon fusarium wilt and enhancing seedling growth,contributing to the sustainable and efficient production of watermelon and providing a foundation for the development of biological control agents against this disease.关键词
西瓜枯萎病/假单胞菌/生物防治/幼苗促生Key words
watermelon Fusarium wilt/Pseudomonas/biological control/seedling promotion分类
农业科技引用本文复制引用
马倩倩,祁瑞雪,沈婷婷,杨睿祺,熊依玲,马福平,张雪艳..西瓜枯萎病拮抗菌假单胞菌的筛选、鉴定及其防治效果[J].农业科学研究,2025,46(3):17-24,8.基金项目
宁夏冷凉蔬菜高效生产和特色瓜菜种质利用科技创新团队(2024CXTD010) （2024CXTD010）
"长江学者奖励计划"青年学者项目 （）
