农业科学研究2025，Vol.46Issue(3)：36-47,12.
枯草芽孢杆菌对兰州百合干旱胁迫的缓解效应
Alleviating Effect of Bacillus subtilis on Drought Stress in Lilium davidii var.unicolor
摘要
Abstract
This study investigates the alleviating effects of Bacillus subtilis on drought stress in Lilium davidii var.unicolor.Using Lilium davidii var.unicolor as the experimental material,drought stress was simulated with polyethylene glycol(PEG)solutions at four mass concentrations:0％,5％(mild),10％(moderate),and 20％(severe).B.subtilis was applied through root irrigation at concentrations of 0,2×10⁸,4×10⁸,and 8×10⁸ cfu/g,resulting in a total of 16 treatment combinations.Growth traits and physiological indices were measured at the seedling,budding,and flowering stages to identify the optimal treatment for mitigating drought stress.The results indicated that under drought stress,the application of B.subtilis significantly improved key agronomic traits of Lilium davidii var.unicolor,including plant height,stem diameter,basal root number,leaf dimensions,leaf count,and bulb circumference.Significant effects(P<0.05)were particularly noted when 2×10⁸ cfu/g B.subtilis was applied under 5％PEG stress and 4×10⁸cfu/g under 10％PEG stress.At the flowering stage,treatment T9 achieved the highest plant height(33.03 cm),representing a 120.13％increase compared to the control(CK).Treatment T2 yielded the largest stem diameter(5.47 mm),which was 10.06％greater than CK.The maximum leaf count(171)and chlorophyll content(25.53 mg/g)were 3.80 and 2.50 times those of treatment T8,respectively,while T9 exhibited 14.99％more basal roots than the control.Additionally,B.subtilis enhanced antioxidant enzyme activities under drought stress.At the budding stage,CAT activity initially increased and then decreased with rising bacterial concentrations,peaking at 25.97 U/g in T8;SOD activity reached 1 120.10 U/g in T9,which is 3.32 times that of the control;and POD activity decreased with higher bacterial concentrations,with a maximum of 52.27 U/g.Membership function analysis revealed that B.subtilis effectively alleviated mild(5％PEG)and moderate(10％PEG)drought stress,with 2×10⁸ cfu/g being the optimal concentration.This study provides a theoretical foundation for the drought-resistant cultivation of L.davidii var.unicolor.关键词
兰州百合/枯草芽孢杆菌/干旱胁迫/农艺性状/生理活性Key words
Lilium davidii var.unicolor/Bacillus subtilis/drought stress/agronomic traits/physiological activity分类
农业科技引用本文复制引用
宋修泰,陈雨欣,勉鹏飞,张萍,党昕,靳磊..枯草芽孢杆菌对兰州百合干旱胁迫的缓解效应[J].农业科学研究,2025,46(3):36-47,12.基金项目
宁夏回族自治区大学生创新创业训练计划项目(S202510749091) （S202510749091）
国家自然科学基金项目(32260727) （32260727）
宁夏回族自治区重点研发计划项目(2021BBF02025). （2021BBF02025）
