枯草芽孢杆菌对兰州百合干旱胁迫的缓解效应OA
Alleviating Effect of Bacillus subtilis on Drought Stress in Lilium davidii var.unicolor
为探究枯草芽孢杆菌对兰州百合干旱胁迫下生长发育及生理活性的影响,以兰州百合为试验材料,采用0、5％(轻度)、10％(中度)、20％(重度)4 个浓度梯度的PEG溶液模拟干旱胁迫,使用灌根的方法分组依次施加浓度为0、2×108、4×108、8×108 cfu/g枯草芽孢杆菌菌剂,共计16 个处理,通过观测不同时期(幼苗期、现蕾期、开花期)兰州百合生长发育特征,并测定其生理活性指标,筛选缓解干旱胁迫的最佳处理.结果表明:在干旱胁迫下,施用枯草芽孢杆菌菌剂对兰州百合的株高、茎直径、基生根数、横纵径、叶片数、鳞茎周径等农艺性状具有促进作用,尤其在5％PEG溶液和10％PEG溶液处理环境下分别施加2×108和4×108 cfu/g浓度的枯草芽孢杆菌菌剂的效果显著(P<0.05).在开花期,兰州百合在T9处理下的植株最高,为33.03 cm,茎直径的最大值为T2下的5.47 mm,分别较对照高120.13％、10.06％;T10的叶片数最多(171),叶绿素质量比最高(25.53 mg/g),分别是T7的3.80倍和2.50倍;基生根数最大出现在T9处理下,比CK高14.99％.干旱胁迫环境下添加枯草芽孢杆菌菌剂可有效提升兰州百合抗氧化保护酶活性.在现蕾期CAT活性随着菌剂浓度的提高先升后降,于T8处理下达最大值(25.97 U/g);SOD活性于T9下达最大值(1 120.10 U/g),为CK的3.32倍;POD活性随着枯草芽孢杆菌浓度的升高而下降,最大可达52.27 U/g.经隶属函数分析,轻度和中度干旱胁迫下,枯草芽孢杆菌菌剂表现出的缓解作用明显,且以2×108 cfu/g浓度的效果最佳.
This study investigates the alleviating effects of Bacillus subtilis on drought stress in Lilium davidii var.unicolor.Using Lilium davidii var.unicolor as the experimental material,drought stress was simulated with polyethylene glycol(PEG)solutions at four mass concentrations:0％,5％(mild),10％(moderate),and 20％(severe).B.subtilis was applied through root irrigation at concentrations of 0,2×10⁸,4×10⁸,and 8×10⁸ cfu/g,resulting in a total of 16 treatment combinations.Growth traits and physiological indices were measured at the seedling,budding,and flowering stages to identify the optimal treatment for mitigating drought stress.The results indicated that under drought stress,the application of B.subtilis significantly improved key agronomic traits of Lilium davidii var.unicolor,including plant height,stem diameter,basal root number,leaf dimensions,leaf count,and bulb circumference.Significant effects(P<0.05)were particularly noted when 2×10⁸ cfu/g B.subtilis was applied under 5％PEG stress and 4×10⁸cfu/g under 10％PEG stress.At the flowering stage,treatment T9 achieved the highest plant height(33.03 cm),representing a 120.13％increase compared to the control(CK).Treatment T2 yielded the largest stem diameter(5.47 mm),which was 10.06％greater than CK.The maximum leaf count(171)and chlorophyll content(25.53 mg/g)were 3.80 and 2.50 times those of treatment T8,respectively,while T9 exhibited 14.99％more basal roots than the control.Additionally,B.subtilis enhanced antioxidant enzyme activities under drought stress.At the budding stage,CAT activity initially increased and then decreased with rising bacterial concentrations,peaking at 25.97 U/g in T8;SOD activity reached 1 120.10 U/g in T9,which is 3.32 times that of the control;and POD activity decreased with higher bacterial concentrations,with a maximum of 52.27 U/g.Membership function analysis revealed that B.subtilis effectively alleviated mild(5％PEG)and moderate(10％PEG)drought stress,with 2×10⁸ cfu/g being the optimal concentration.This study provides a theoretical foundation for the drought-resistant cultivation of L.davidii var.unicolor.
宋修泰;陈雨欣;勉鹏飞;张萍;党昕;靳磊
宁夏大学 林业与草业学院,宁夏 银川 750021宁夏大学 林业与草业学院,宁夏 银川 750021宁夏科育种苗有限公司,宁夏 银川 750001宁夏大学 林业与草业学院,宁夏 银川 750021宁夏大学 林业与草业学院,宁夏 银川 750021宁夏大学 林业与草业学院,宁夏 银川 750021
农业科技
兰州百合枯草芽孢杆菌干旱胁迫农艺性状生理活性
Lilium davidii var.unicolorBacillus subtilisdrought stressagronomic traitsphysiological activity
《农业科学研究》 2025 (3)
36-47,12
宁夏回族自治区大学生创新创业训练计划项目(S202510749091)国家自然科学基金项目(32260727)宁夏回族自治区重点研发计划项目(2021BBF02025).
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