山东医药2025，Vol.65Issue(9)：35-39,5.DOI:10.3969/j.issn.1002-266X.2025.09.008
巨噬细胞-肌成纤维细胞转化在急性肺损伤后早期肺纤维化中的作用机制
Role of macrophage-to-myofibroblast transition in early pulmonary fibrosis induced by acute lung injury
胡胜娟 1胡蝶 2吴磊3
作者信息
- 1. 武汉科技大学医学院公共卫生学院劳动卫生与环境卫生学教研室,湖北 武汉 430065||职业危害识别与控制湖北省重点实验室,湖北 武汉 430065||鄂州市中心医院儿科,湖北 鄂州 436000
- 2. 武汉科技大学医学院公共卫生学院劳动卫生与环境卫生学教研室,湖北 武汉 430065||职业危害识别与控制湖北省重点实验室,湖北 武汉 430065||武汉大学人民医院呼吸与危重症医学科,湖北 武汉 430060
- 3. 武汉科技大学医学院公共卫生学院劳动卫生与环境卫生学教研室,湖北 武汉 430065||职业危害识别与控制湖北省重点实验室,湖北 武汉 430065
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摘要
Abstract
Objective To investigate the mechanism of action of macrophage-to-myofibroblast transition(MMT)on early pulmonary fibrosis induced by acute lung injury.Methods Eighteen SPF healthy mice were divided into the model group and control group.Mice in the model group were treated by lipopolysaccharide(LPS)to establish the early pulmo-nary fibrosis models,while mice in the control group received tracheal and intraperitoneal injections of equal volume of nor-mal saline at the same time points.On day 3,7,and 10 after modeling,lung tissue samples were collected from the mice.The extent of pulmonary fibrosis was assessed using the Ashcroft score.The number of myofibroblasts exhibiting MMT phe-notype[co-labeled with CD68 and α-smooth muscle actin(α-SMA)]in the lung tissues was measured by immunofluores-cence staining.The protein levels of mothers against decapentaplegic homolog 3(Smad3)and transforming growth factor-β1(TGF-β1)in the mouse lung tissues were detected by Western blotting and ELISA,respectively.Results Compared with the control group,the Ashcroft score significantly increased in the model group on day 7 and 10(all P<0.05).The relative protein expression levels of Smad3 and TGF-β1 in the lung tissues of the model group were significantly higher than those in the control group on day 7 and 10 after modeling(all P<0.05).In vitro,the protein expression levels of Smad3 and α-SMA,and the mRNA expression levels of α-SMA,Fibronectin,and Collagen-I in the intervention group in-creased significantly over time(all P<0.05).Conclusions As the duration of injury increases,the proportion of MMT-phenotype cells in the lung tissues of mice with acute lung injury gradually increases.MMT-phenotype cells may contrib-ute to the development and progression of pulmonary fibrosis in injured mice by regulating the TGF-β1/Smad3 signaling pathway.关键词
巨噬细胞/成纤维细胞/巨噬细胞-肌成纤维细胞转化/肺损伤/肺纤维化Key words
macrophage/fibroblast/macrophage-to-myofibroblast transition/lung injury/pulmonary fibrosis分类
医药卫生引用本文复制引用
胡胜娟,胡蝶,吴磊..巨噬细胞-肌成纤维细胞转化在急性肺损伤后早期肺纤维化中的作用机制[J].山东医药,2025,65(9):35-39,5.
