热带亚热带植物学报2025，Vol.33Issue(5)：555-565,11.DOI:10.11926/jtsb.4938
多花黄精种子解除休眠和萌发的代谢产物变化机制
Changes of Metabolites in Dormancy Release and Germination of Polygonatum cyrtonema Seeds
摘要
Abstract
The seeds of Polygonatum cyrtonema have a comprehensive dormancy,which seriously restricts its production and industrial development.The seed dormancy was broken by using warm sand storage treatment,and the changes in metabolomics,transcriptomics at six stages of dormancy release and germination were analyzed.The results showed that amino acid metabolism and synthesis played a key role in the process of dormancy release and germination of P.cyrtonema seeds.The correlation between five metabolites(L-threonine,L-glutamic acid,indole,S-adenosylmethionine and choline)and ten differentially expressed genes in the amino acid metabolism and synthesis related pathways was analyzed.Adenosine diphosphate,alanine-tryptophan,coumarin 151,loniceroside,N-methylthreonine and sedoheptulose-7-phosphate were identified as important characteristic molecules for the germination of P.cyrtonema seeds,and ten related genes were also identified.The expression trends of some genes were verified by qPCR.The key metabolic pathways and gene regulatory networks were revealed for the release of seed dormancy of P.cyrtonema from the levels of metabolite changes and molecular mechanisms,which would provide a theoretical basis for further understanding the molecular mechanism of seed dormancy release and optimizing the germination conditions of P.cyrtonema seeds.关键词
多花黄精/沙藏/种子休眠/萌发/代谢组学/转录组学Key words
Polygonatum cyrtonema/Sand storage/Seed dormancy/Germination/Metabolomics/Transcriptomics引用本文复制引用
牛雨晴,朱育菁,郑梅霞,朱雁鸣,陈宏,黄贤贵,苏海兰..多花黄精种子解除休眠和萌发的代谢产物变化机制[J].热带亚热带植物学报,2025,33(5):555-565,11.基金项目
福建省属公益项目(2023R1032003) （2023R1032003）
福建省重大科研专项(2022NZ029017) （2022NZ029017）
福建省农科院英才项目(YC2021002) （YC2021002）
福建省农科院科技创新及服务团队项目(2022KF01-1)资助 This work was supported by the Project for Public Welfare in Fujian(Grant No.2023R1032003),the Project for Major Scientific Research in Fujian(Grant No.2022NZ029017),the Project for Talent Development at Fujian Academy of Agricultural Sciences(Grant No.YC2021002),and the Project for Scientific Innovation and Service Teams at Fujian Academy of Agricultural Sciences(Grant No.2022KF01-1). （2022KF01-1）
