热带亚热带植物学报2025，Vol.33Issue(5)：566-576,11.DOI:10.11926/jtsb.4930
盾叶秋海棠基因组微型反向重复转座子MITEs的分布模式和表达分析
Distribution of MITEs(Miniature Inverted-repeat Transposable Elements)and Their Expression Patterns in the Genome of Begonia peltatifolia
摘要
Abstract
By using the published genome of Begonia peltatifolia and the transcriptome data of different tissues,the miniature inverted repeat transposable elements(MITEs)in the genome were identified and classified,and their distribution characteristics,insertion preferences and expression states in the genome were analyzed.The results showed that a total of 925 MITEs copies were identified,belonging to four superfamilies:hAT,Mutator,PIF/Harbinger and Tc1/Mariner,among which the hAT family accounted for the highest proportion,reaching 70.49%.The analysis of insertion positions revealed that 59.5%of the MITEs elements were located in the flanking regions(upstream and downstream 5 kb,respectively)or within genes,while the other approximately 40%of the MITEs were located in telomeres,centromeres or other gene-sparse regions;moreover,the number of MITEs copies located in the 5' flanking region of genes was approximately twice that in the 3' flanking region,and the insertion hotspots were within 1000 to 3000 base pairs.These co-transcribed genes were mainly related to secondary metabolism,energy synthesis and transport functions;those inserted into the genes were mainly distributed in non-coding regions(UTRs and introns),and no MITEs insertions were observed in exons.The functional enrichment of the genes with MITEs insertions was mainly related to functions,protein synthesis and the expression of genetic information.A total of 68 MITEs co-transcriptions were detected in the transcriptome data of different tissues of B.peltatifolia,with very little expression in vegetative organs such as pedicels and leaves.They were expressed more in reproductive organs such as flowers and active meristems of roots.Among the transcripts containing MITEs,80.8%had lower expression levels or no significant difference compared with their isoforms,while only 19.1%had increased expression levels.Therefore,these would lay the foundation for studying the epigenetic regulation mechanism of transposons in drought tolerance and provides genetic resources for the breeding of drought-tolerant Begonia varieties.关键词
盾叶秋海棠/MITEs转座子/基因插入偏好/基因功能Key words
Begonia peltatifolia/MITEs/Gene insertion preference/Gene function引用本文复制引用
黄泽铖,郭微,王龙远,李凌飞,吴伟..盾叶秋海棠基因组微型反向重复转座子MITEs的分布模式和表达分析[J].热带亚热带植物学报,2025,33(5):566-576,11.基金项目
岭南特色园林观赏植物种质创新与开发应用创新团队项目(2023KCXTD017) （2023KCXTD017）
广东省现代农业产业技术体系创新团队建设项目(2024CXTD12) （2024CXTD12）
深圳市城管局科研项目(202408)资助 This work was support by the Project for Lingnan Characteristic Garden Ornamental Plant Germplasm Innovation,Development and Application Innovation Team(Grant No.2023KCXTD017),the Project for Modern Agricultural Industry Technology System Innovation Team Construction in Guangdong(Grant No.2024CXTD12),and the Project for Scientific Research of Shenzhen Urban Management Bureau(Grant No.202408). （202408）
