虾青素对赤点石斑鱼肝细胞和肠上皮细胞结构和自噬的影响
Effects of astaxanthin on the structure and autophagy of hepatocytes and intestinal epithelial cells of Epinephelus akaara
摘要
Abstract
Cultured Epinephelus akaara generally suffers from slow growth and decreased skin beauty.It has been previously verified that astaxanthin,as a feed additive,has a positive effect on the coloring and antioxidant capacity of E.akaara.Other studies have found that astaxanthin can induce autophagy to regulate the body's lipid metabolism and promote liver and intest-inal health functions.Therefore,this study conducted a three-month experiment on E.akaara fed with astaxanthin supplemen-ted with different concentrations(0,100,200,400,and 800 mg/kg).The effects of astaxanthin on HSI,hepatocyte and intest-inal epithelial cell structure and autophagy of E.akaara were investigated by measuring hepatosomatic index(HSI)and using transmission electron microscopy(TEM).The regression analysis model showed that with the increase of astaxanthin supple-mental level,liver HSI of E.akaara showed a decreasing trend,and HSI in AST800 group was significantly lower than that in control group(P＜0.05).The ultrastructure of liver showed that the area of hepatocytes in AST800 groupwas significantly smal-ler than that of the control group(P＜0.05),and the number of autophagosomes,autophagolysosomes and mitochondria increased significantly(P＜0.05),while the number and area of lipid droplets were significantly decreased(P＜0.05).The pres-ence of small clumpy lipid droplets and mitochondria in the autophagosome contents suggested the occurrence of lipid droplet autophagy and mitochondrial autophagy.The intestinal ultrastructure showed that the number of autophagosomes,autolyso-somes and mitochondria in intestinal mucosal epithelial cells of AST800 group was significantly increased compared with the control group(P＜0.05),and the microvilli were more densely arranged,and the structure of tight junction between cells became longer.Studies have shown that adding astaxanthin to the diet can induce autophagy,regulate cellular lipid metabolism,reduce HSI,phagocytic mitochondria with abnormal structure,inhibit oxidative stress,and promote intestinal barrier function by regu-lating cell tight junction.In conclusion,autophagy plays an important role in astaxanthin regulation of liver lipids and promo-tion of intestinal barrier function in E.akaara,which is beneficial to its physiological activities.At the same time,the results of this study suggest that lipid autophagy may be a new target for astaxanthin to improve the accumulation of excess fat in the liver caused by dietary diet,and provide experimental basis for further development and utilization of astaxanthin in fish feed.关键词
赤点石斑鱼/虾青素/肝体指数/肝细胞/肠上皮细胞/自噬/脂质代谢Key words
Epinephelus akaara/astaxanthin/hepatosomatic index/hepatocyte/intestinal epithelial cell/autophagy/lipid meta-bolism分类
农业科技引用本文复制引用
苏金枝,田永胜,李胜忠,邓远,李振通,黎琳琳,王林娜,刘阳,王清滨,赵侠,孙芳芳..虾青素对赤点石斑鱼肝细胞和肠上皮细胞结构和自噬的影响[J].水产学报,2025,49(11):61-71,11.基金项目
国家重点研发计划(2022YFD2400502) （2022YFD2400502）
泰山产业领军人才项目(LJNY202109) （LJNY202109）
山东省重点研发项目(2022LZGC016) （2022LZGC016）
海南省重点研发项目(ZDYF2022XDNY243) （ZDYF2022XDNY243）
青岛市自然科学基金(23-2-1-53-zyyd-jch) （23-2-1-53-zyyd-jch）
财政部和农业农村部:国家现代农业海水鱼产业技术体系专项(CARS-47-G31) （CARS-47-G31）
中央级公益性科研院所基本科研业务费专项(2020TD19) （2020TD19）
黄海水产研究所科研经费(20603022021010) （20603022021010）
青岛博士后应用研究项目 National Key Research and Development Program of China(2022YFD2400502) （2022YFD2400502）
Taishan Industry Leading talent Project(LJNY202109) （LJNY202109）
Key Research and Development Project of Shandong Province(2022LZGC016) （2022LZGC016）
Key Research and Development Project of Hainan Province(ZDYF2022XDNY243) （ZDYF2022XDNY243）
Qingdao Natural Science Foundation(23-2-1-53-zyyd-jch) （23-2-1-53-zyyd-jch）
Ministry of Finance and Ministry of Agriculture and Rural Affairs:National Modern Agricultural Marine Fish Industry Technology System Special Funding Project(CARS-47-G31) （CARS-47-G31）
Central Public-interest Scientific Institute Basal Research Fund,CAFS(2020TD19) （2020TD19）
Yellow Sea Fisheries Research Institute Research Fees(20603022021010) （20603022021010）
Qingdao Postdoctoral Applied Research Project （）
