水产学报2025，Vol.49Issue(11)：72-87,16.DOI:10.11964/jfc.20240314434
急性碱胁迫对金钱鱼肝脏和鳃组织结构及转录组响应的影响
Effects of acute alkali stress on the structural and transcriptomic responses of liver and gill tissues in Scatophagus argus
摘要
Abstract
To elucidate the response mechanisms of Scatophagus argus to carbonate alkalinity stress,we established a control group in freshwater and an experimental group exposed to 30 mmol/L carbonate alkalinity.Liver and gill tissues were collected at 0,3,6,and 9 hours post-stress for histological examination and transcriptome sequencing.Histological analysis revealed that under alkaline stress,liver cells exhibited varying degrees of atrophy and vacuolization,while gill tissues demonstrated damage characterized by epithelial cell shedding,gill lamella deformation,and larger spacing.Transcriptome sequencing after 9 hours of alkaline stress identified 270 differentially expressed genes(DEGs)in liver tissue,with 190 genes up-regulated and 80 down-regulated.These DEGs were primarily associated with pathways related to energy synthesis and metabolism,including cyto-chrome P450-related metabolism,adipocytokine signaling,and steroid metabolism.Notably,the up-regulation of hsp70 and hbb1,alongside the down-regulation of gpx1,indicated the activation of oxidative stress response mechanisms.In gill tissue,114 DEGs were identified,with 39 up-regulated and 75 down-regulated,affecting pathways such as cell adhesion,steroid bio-synthesis,and PPAR signaling.The significant down-regulation of prkca and rab5a suggested a potential compromise in cell adhesion capacity.In addition,the common differentially expressed genes ck and nlrp12 in liver and gill tissues play a key role in immune defense.These findings indicate that the adaptation of S.argus to carbonate alkalinity stress involves structural and functional alterations in liver and gill tissues,along with the regulation of gene expression related to energy balance and immune response.This study provides critical insights into the molecular mechanisms underlying alkali resistance in S.argus.关键词
金钱鱼/碱胁迫/组织结构/转录组测序/氧化应激Key words
Scatophagus argus/alkali stress/organizational structure/transcriptome sequencing/oxidative stress分类
生物科学引用本文复制引用
杨磊,李广丽,田昌绪,彭友琦,刘勇,陈宇,段存煜,韩彤,施钢,朱春华..急性碱胁迫对金钱鱼肝脏和鳃组织结构及转录组响应的影响[J].水产学报,2025,49(11):72-87,16.基金项目
新疆维吾尔自治区重点研发任务专项(2024B02014-3) （2024B02014-3）
湛江市科技计划项目(2022A01046) （2022A01046）
国家自然科学基金(32172971) （32172971）
广东省自然科学基金(2021A1515010430) （2021A1515010430）
广东海洋大学本科生创新团队项目(CXTD2023003) （CXTD2023003）
广东省南美白对虾现代种业产业园项目(K22221) Key Research and Development Task of the Xinjiang Uygur Autonomous Region(2024B02014-3) （K22221）
Zhanji-ang Science and Technology Plan Project(2022A01046) （2022A01046）
National Natural Science Foundation of China(32172971) （32172971）
Guang-dong Basic and Applied Basic Research Foundation(2021A1515010430) （2021A1515010430）
Undergraduate Innovation Team Project of Guang-dong Ocean University(CXTD2023003) （CXTD2023003）
Modern Seed Industry Park for Whiteleg Shrimp of Guangdong Province(K22221) （K22221）
