水产学报2025，Vol.49Issue(11)：88-101,14.DOI:10.11964/jfc.20240314437
温度和摄食水平对三角帆蚌消化、代谢酶活性及基因表达的影响
Enzyme activities and gene expression in digestion and metabolism of the triangle sail mussel(Hyriopsis cumingii)under different temperature and diet ration
摘要
Abstract
The traditional culture method of Hyriopsis cumingii is in urgent need of transformation.Industrial aquaculture,a sustainable cultivation mode,relies on the control of environmental conditions and food supply.To evaluate combined effects of different rations(high-ration,medium-ration,and low-ration)and temperatures[(13.0±0.2),(23.0±0.2)and(33.0±0.2) ℃)]on digestion and metabolism of H.cumingii,a comprehensive 3 × 3 test factorial experiment was established.Digestive enzymes(e.g.,amylase,lipase,cellulase)and metabolic enzymes(e.g.,glutamic pyruvate transaminase,glutamic oxaloacetate transam-inase,pyruvate kinase,hexokinase)along with metabolism-related genes(e.g.,CPT1,LDLR)were assayed over a 21-day period in mussels.The results showed that levels of digestive and metabolic indicators remained stable in the medium-ration×23 ℃ treatment,and were significantly higher than others on day 21(P＜0.05).When exposed to low or high-ration,as well as 13 or 33 ℃,these biochemical indicators rose briefly and then significantly declined(P＜0.05).Principal component ana-lysis(PCA)demonstrated a clear separation of treatment groups based on digestive and hepatic enzyme activities,with PC1 accounting for 67.73％-80.73％of the total variance.Enzyme activities were notably reduced under extreme temperatures and altered feeding levels,while lipase,amylase,cellulase,and key hepatic metabolic enzymes contributed positively to PC1.These findings suggest that the medium-ration and a temperature of 23 ℃ were the optimal diet quantity and environmental conditions for H.cumingii.Short-term thermal,cold,semi-starving,and overfeeding treatments induced metabolic levels of mussels to meet the energy demand for anti-oxidative stress.However,long-term stress led to liver injury of H.cumingii,resulting in reduced digestive and metabolic efficiency of the mussel,and subsequently affecting its growth and overall health.This study holds practical significance in exploring culture environment and feeding conditions,controlling breeding cost,and increasing growth and yield in industrial aquaculture.关键词
三角帆蚌/温度/摄食水平/消化/代谢Key words
Hyriopsis cumingii/temperature/ration level/digestion/metabolism分类
农业科技引用本文复制引用
李捷,孙瑜,刘炀,姚维志,腾树君,李艳红,吴正理..温度和摄食水平对三角帆蚌消化、代谢酶活性及基因表达的影响[J].水产学报,2025,49(11):88-101,14.基金项目
重庆市自然科学基金(CSTB2022NSCQ-MSX0305) （CSTB2022NSCQ-MSX0305）
现代农业产业技术体系(CARS-49) （CARS-49）
重庆市生态渔业产业技术体系(CQMAITS202315) （CQMAITS202315）
重庆市水产科技重点攻关项目(CQFTIU2024-09) Chongqing Natural Science Foundation Project(CSTB2022NSCQ-MSX0305) （CQFTIU2024-09）
China Agriculture Research System(CARS-49) （CARS-49）
Chongqing Ecological Fisheries Industry Technology System(CQMAITS202315) （CQMAITS202315）
Chongqing Key Fisher-ies Science and Technology Project(CQFTIU2024-09) （CQFTIU2024-09）
