热带作物学报2025，Vol.46Issue(10)：2314-2322,9.DOI:10.3969/j.issn.1000-2561.2025.10.003
木薯碳酸酐酶CA基因的克隆和功能研究
Cloning and Functional Research of Carbonic Anhydrase CA Gene in Cassava
摘要
Abstract
Carbonic anhydrase(CA)plays an important role in the photosynthesis of plants and response to stress.Cas-sava CA gene was cloned using the cDNA of SC124 as template.The CDS length of MeCA was 1008 nt,encoding 336 amino acids.The homology was 99.70％between the cloned sequence and Manes.15G167500.1 in the database.The evolutionary tree resulted that MeCA belonged to the same branch as the AtβCA subfamily of Arabidopsis.The sequence similarity between MeCA and AtβCA1 protein reached 73.13％and contained identical motif.MeCA gene expression was the highest in functional leaves,followed in young leaves,significantly higher in fibrous roots and stems,and sig-nificantly down-regulated in shading stressed cassava leaves.In addition,the gene showed significant upregulated ex-pression in the initial stage,and then it dropped to the control level when cassava leaves were treated with low tem-perature.This study created transgenic cassava with overexpression of MeCA gene,and observed that MeCA protein was localized in chloroplasts,and the contents of various chlorophyll were significantly higher in the leaves of trans-genic plants than that of control.The results of yeast two-hybrid point-to-point experiments showed that MeCA protein interacted with MeH1.2.The result indicated that MeCA gene could respond to light and low temperature stresses,and maight participate in plant response to stress by affecting plant growth interacting with MeH1.2 proteins.This study would provide a new gene resource for genetic improvement of cassava resistance.关键词
CA基因/木薯/抗逆/叶绿素含量Key words
CA gene/cassava/stress resistance/chlorophyll content分类
农业科技引用本文复制引用
王梦月,李文彬,张秀春,于晓玲,阮孟斌,赵平娟..木薯碳酸酐酶CA基因的克隆和功能研究[J].热带作物学报,2025,46(10):2314-2322,9.基金项目
海南省自然科学基金项目(No.321RC1095,No.323RC538) （No.321RC1095,No.323RC538）
国家自然科学基金面上项目(No.32472190). （No.32472190）
