热带作物学报2025，Vol.46Issue(10)：2323-2334,12.DOI:10.3969/j.issn.1000-2561.2025.10.004
茶树CAD基因家族的鉴定与表达分析及小贯小绿叶蝉为害对其表达的影响
Identification and Expression Analysis of the CAD Gene Family in Tea Plant(Camellia sinensis)and Their Response to Infestation by Empoasca onukii
摘要
Abstract
During the biosynthesis process of lignin in plants,cinnamyl alcohol dehydrogenase(CAD)plays a crucial role,catalyzing the final step reaction in the entire metabolic pathway.To explore the potential functions of the CAD gene family in tea plants,CAD gene family members were identified in the genome of Huangyan tea plants and a series of bioinformatics analyses were conducted.Based on transcriptome data,the gene expression of the members in differ-ent organs of tea plants and after damage by the Empoasca vitis were studied.A total of 36 members of HD-CsCADs were identified,which were unevenly distributed on 9 chromosomes and encoded amino acid lengths ranging from 300 aa to 621 aa and protein molecular weights ranging from 321.40 kDa to 666.49 kDa.HD-CsCADs had 0 to 3 introns and the promoters containing 79 types of cis-acting elements,among which the number of elements related to stress re-sponse was the highest.Combined with the phylogenetic tree,HD-CsCADs could be divided into 4 subfamilies.There were significant differences in the expression levels of HD-CsCADs in different organs of tea plants.HD-CsCAD-15 was highly homologous to CAD genes involved in lignin biosynthesis in other plants and was highly expressed in stem tissues.In addition,the expression of the genes after damage by E.vitis on tea seedlings was analyzed.The expression of HD-CsCAD-11 and HD-CsCAD-15 was relatively obvious and could be used as important CAD genes related to lig-nin metabolism and tea plant defense against pests.The results would provide theoretical basis for the defense mecha-nism of tea plants against the small green leafhopper and the utilization of CAD gene functions.关键词
茶树/肉桂醇脱氢酶基因/基因家族/木质素/小贯小绿叶蝉Key words
tea plant/CAD gene/gene family/lignin/Empoasca vitis分类
农业科技引用本文复制引用
杨欢,阮玲玲,闫佳伟,任卫威,柳紫琼,金珊..茶树CAD基因家族的鉴定与表达分析及小贯小绿叶蝉为害对其表达的影响[J].热带作物学报,2025,46(10):2323-2334,12.基金项目
中国乌龙茶产业协同创新中心开放研究基金项目(No.2024W02) （No.2024W02）
福建张天福茶叶发展基金会科技创新基金项目(No.FJZTF01). （No.FJZTF01）
