热带作物学报2025，Vol.46Issue(10)：2335-2345,11.DOI:10.3969/j.issn.1000-2561.2025.10.005
番杏类甜蛋白基因TtTLP11超表达的转基因拟南芥对非生物胁迫的抗性功能分析
Functional Analysis of a Tetragonia tetragonoides(Pall.)Kuntze Thaumatin-Like Protein Gene,TtTLP11,in Transgenic Overexpression Arabidopsis thaliana involved in Abiotic Stress Tolerance
摘要
Abstract
Tetragonia tetragonoides(Pall.)Kuntze is a seawater vegetable with strong tolerance to multiple abiotic stresses.Plant thaumatin-like proteins(TLPs)are involved in various biological and abiotic stress responses.In order to elucidate the possible roles of a T.tetragonoides thaumatin-like protein gene(TtTLP11)in abiotic stress resistance,we cloned this gene and performed transgenic over-expression assay in Arabidopsis thaliana,then the homozygotic trans-genic lines were obtained and performed subsequent analysis for stresses tolerance.In this study,the seeds from trans-genic plants over-expressing TtTLP11 and wild type Arabidopsis plants were challenged with high salinity,high osmotic stress,heat and mixed salt-alkali stresses,by detecting the seed germinating rates,seedling growth status,thereby as-sessing the stress resistance of these transgenic Arabidopsis plants.The purpose of this study was to clarify the abiotic stress resistance functions of the TtTLP11 and the possible molecular mechanisms.The results showed that under vari-ous abiotic stress conditions,the over-expression of TtTLP11 could alleviate the inhibition effects of seed germination and relieve the environmental damage to seedlings,thereby improving the abiotic stress tolerance of transgenic plants.It is speculated that TtTLP11 might alleviate dramatic changes in osmotic pressure in plants and maintain water homeosta-sis,and then affect the stress tolerance of plants.The results could provide a theoretical basis for further analysis of the molecular regulatory network of plant response to alleviate abiotic stress damage.关键词
番杏/类甜蛋白基因/非生物胁迫/转基因拟南芥Key words
Tetragonia tetragonoides(Pall.)Kuntze/TLP/abiotic stresses/transgenic Arabidopsis分类
农业科技引用本文复制引用
黄增旺,谢富英,简曙光,黎庭耀,张美..番杏类甜蛋白基因TtTLP11超表达的转基因拟南芥对非生物胁迫的抗性功能分析[J].热带作物学报,2025,46(10):2335-2345,11.基金项目
广东省科技计划项目(No.2024B1212050007). （No.2024B1212050007）
