热带作物学报2025，Vol.46Issue(10)：2355-2363,9.DOI:10.3969/j.issn.1000-2561.2025.10.007
芥蓝几丁质酶基因家族对环境信号的响应分析
Analysis of Response of Chitinase Gene Family to Environmental Signals in Chinese Kale
摘要
Abstract
Chitinase plays a crucial role in plant stress resistance and growth development.To elucidate the effects of plant hormones and environmental stress on the expression of the mustard chitinase gene,this study investigated three plant hormones-salicylic acid(SA),1-amino-cyclopropane-1-carboxylic acid(ACC),and methyl jasmonate(MeJA)—and the influence on the transcriptional regulation of the chitinase gene family in Brassica oleracea cv.BaiHua.Addi-tionally,seedlings were subjected to two stress conditions:infection by Pectobacterium carotovorum subsp.Brasiliense and exposure to high temperature.The transcriptional levels of the Chinese kale chitinase gene family members were analyzed using the quantitative real-time polymerase chain reaction(qRT-PCR)method.Results demonstrated that among the 18 genes within the chitinase gene family of Chinese mustard,16 exhibited significant induction under soft rot fungal infection,while 8 responded to high-temperature stress.Furthermore,all 18 genes showed responsiveness to plant hormone-induced expression.This study highlights the potential role of the chitinase gene in mediating responses to hormonal signals and environmental stress,thereby contributing to stress resistance and growth development in plants.The findings would provide valuable insights for future molecular breeding strategies targeting Chinese kale.关键词
芥蓝/几丁质酶基因/植物激素/抗逆机理/qRT-PCRKey words
Chinese kale/chitinase genes/plant hormones/mechanism of stress resistance/qRT-PCR分类
农业科技引用本文复制引用
曾语,吴卉芳,邱昱岑,于雪晴,朱俊杰,谢炳峰,吕美玲..芥蓝几丁质酶基因家族对环境信号的响应分析[J].热带作物学报,2025,46(10):2355-2363,9.基金项目
福建省自然科学基金项目(No.2022J01589) （No.2022J01589）
国家级大学生创新训练项目(No.202410389011). （No.202410389011）
