实用医学杂志2025，Vol.41Issue(19)：3000-3007,8.DOI:10.3969/j.issn.1006-5725.2025.19.007
肠道菌群失调与青春期社交隔离小鼠认知障碍的关联性及关键致病菌群鉴定
Gut microbiota dysbiosis in adolescent socially isolated mice with cognitive impairment and identification of key pathogenic microbial communities
摘要
Abstract
Objective To elucidate the role of gut microbiota in cognitive impairment induced by adolescent social isolation(ASI)and identify key pathogenic microbial communities.Methods Sixteen male C57BL/6 mice(postnatal days 21～23)were randomly assigned to a control group(group-housed,4/cage,n=8)or an ASI group(single-housed,1/cage,n=8).After 5 weeks of ASI(5-ASI),fecal samples underwent 16S rRNA sequencing for microbiota analysis.Cognitive behavior was assessed using open field,Y-maze,novel object recognition tests.Correlation analysis integrated microbial abundance with behavioral parameters to screen cognition-associated taxa.Results(1)Behavior:5-ASI mice showed unchanged locomotion(open field)but significantly reduced spontaneous alternation(Y-maze,P<0.01)and lower cognitive index(novel object recognition,P<0.001),confirming spatial and recognition memory deficits.(2)Microbiota:5-ASI mice exhibited decreased α-diversity(P<0.05)and altered β-diversity(P<0.001).LEfSe analysis revealed depleted taxa(e.g.,Prevotellaceae_NK3B31_group,Tyzzerella,Allobaculum,Ruminococcus)and enriched taxa(e.g.,Butyricicoccus,Lachnoclostridium).(3)Correlations:Positive associations with cognitive performance were observed for Prevotellaceae_NK3B31_group,Allobaculum,and Ruminococcus(P<0.05),while Butyricicoccus and Lachnoclostridium showed negative correlations(P<0.05).Conclusion Adolescent social isolation induces spatial/recognition memory impairment and gut microbiota dysbio-sis.Specific microbial alterations(Prevotellaceae_NK3B31_group,Butyricicoccus)correlate with cognitive deficits,providing novel insights into microbiota-mediated mechanisms and potential therapeutic targets.关键词
青春期/社交隔离/认知障碍/肠道菌群Key words
adolescence/social isolation/cognitive impairment/gut microbiota分类
医药卫生引用本文复制引用
敬金洲,李泽林,罗争意,胡玉莹..肠道菌群失调与青春期社交隔离小鼠认知障碍的关联性及关键致病菌群鉴定[J].实用医学杂志,2025,41(19):3000-3007,8.基金项目
国家自然科学基金项目(编号:32170953) （编号:32170953）
广东省自然科学基金项目(编号:2023A1515010165,2024A1515010966) （编号:2023A1515010165,2024A1515010966）
