实用医学杂志2025，Vol.41Issue(19)：3026-3033,8.DOI:10.3969/j.issn.1006-5725.2025.19.010
PCBP1在胃癌中的表达及其与铁死亡因子STUB1的关系
The expression of PCBP1 in gastric cancer and its relationship with ferroptosis factor STUB1
摘要
Abstract
Objective To investigate the expression characteristics of poly(rC)-binding protein 1(PCBP1)in gastric cancer tissues and their clinical significances by bioinformatics analysis combined with experimental verification,and to identify its relationship with STIP1 homology and U-Box containing protein 1(STUB1).Specifically,this study aims to verify the expression patterns of PCBP1 and STUB1 in gastric cancer and determine their relationships with clinicopathological features by immunohistochemistry to provide a theoretical framework as well as potential intervention strategies for gastric cancer.Methods Data of PCBP1 expression in gastric cancer and adjacent tissues were obtained from TIMER 2.0 online analysis website.KEGG pathway enrichment analysis was performed using gastric cancer data(STAD)in the TCGA(the Cancer Genome Atlas)database,and its potential mechanism was determined.The main regulatory factor STUB1 was found in the fer-roptosis regulatory pathway.Subsequently,PCBP1 and STUB1 expressions in 33 cases of gastric cancer tissues and corresponding adjacent tissues were detected by immunohistochemistry.The collected cases were grouped according to different degrees of differentiation,age,gender,tumor size,depth of tumor invasion,TNM stage and pathological morphology.The positive expression rates of PCBP1 and STUB1 were observed.The correlation between the two proteins and the correlation between clinical and pathological features were analyzed by c2 test.Finally,the relationship between PCBP1 and STUB1 and malignancy of gastric cancer was further explored.Results Immunohistochemical results showed that the positive expression rate of PCBP1 in cancer tissues was 69.7%,which was significantly higher than that in adjacent tissues(48.5%).The positive expression rate of STUB1 in cancer tissues was 39.4%,which was lower than that in adjacent tissues(54.5%),statistically significant difference(P<0.05).The positive expression rate of PCBP1 was correlated with tumor differentiation,lymph node metastasis and Lauren classification(P<0.05),but not with patient's age,gender,depth of inva-sion,clinical stage,nerve infiltration,and intravascular tumor thrombus(P>0.05).The positive expression rate of STUB1 was correlated with tumor differentiation,depth of invasion,lymph node metastasis and Lauren classification(P<0.05).The Spearman correlation coefficient between PCBP1(cancer)and STUB1(cancer)was-0.413,with P=0.017(P<0.05),indicating that there was a significant negative correlation between them.Conclusion PCBP1 participates in the malignant progression of gastric cancer by regulating the main regulator STUB1 in the ferroptosis pathway.Theoretically,it provides a new insight into molecular mechanism as well as a potential therapeutic strategy for treating gastric cancer.关键词
胃癌/PCBP1/STUB1/生物信息学/铁死亡/免疫组化Key words
gastric cancer/PCBP1/STUB1/bioinformatics/iron death/immunohistochemistry分类
医药卫生引用本文复制引用
卢旭满,史正一,雷元睿,黄海滨,邓仁淼,董旭东,黄宇亮,孔凡彪,王晓通..PCBP1在胃癌中的表达及其与铁死亡因子STUB1的关系[J].实用医学杂志,2025,41(19):3026-3033,8.基金项目
国家自然科学基金项目(编号:81660416,82260468) （编号:81660416,82260468）
广西自然科学基金项目(编号:2023GXNSFAA026341,2023GXNS-FAA026143) （编号:2023GXNSFAA026341,2023GXNS-FAA026143）
广西适宜技术推广项目(编号:S2024006) （编号:S2024006）
