时珍国医国药2025，Vol.36Issue(19)：3615-3624,10.DOI:10.70976/j.1008-0805.SZGYGY-2025-1903
强志方对抽动障碍共患注意力缺陷多动障碍大鼠的疗效及潜在机制研究
Study on the therapeutic effect and potential mechanism of Qiangzhi Formula(强志方)in rats with tic disorder comorbid with attention deficit hyperactivity disorder
摘要
Abstract
Objective To evaluate the therapeutic effects and mechanisms of Qiangzhi Formula(强志方,QZF)in a rat model of tic disorder(TD)combined with attention deficit hyperactivity disorder(ADHD),providing experimental data and theoretical basis for the prevention and treatment of TD combined with ADHD.Methods Six Sprague-Dawley(SD)rats were designated as the blank control group,while 30 spontaneously hypertensive rats(SHR)were randomly divided into the sham surgery group,model group,and low/medium/high-dose QZF groups.Behavioral changes were assessed using stereotypy scores,locomotor scores,open field test(OFT),elevated plus maze test(EPMT),and Morris water maze test(MWMT).Western blot(WB)and RT-PCR were used to detect the expression levels of calcium/calmodulin-dependent protein kinase Ⅱ(CaMK Ⅱ),tyrosine hydroxylase(TH),and dopamine(DA)-related proteins and mRNA.Results Stereotypy and locomotor scores were significantly increased in the model group but decreased notably after QZF treatment,with the most significant improvement observed in the high-dose group.Behavioral tests showed significant improvements in locomotor indicators in OFT and EPMT in the high-dose group,as well as shortened escape latency and increased target quadrant-related indicators in the MWMT.Western blot(WB)and RT-PCR revealed that compared to the model group,the high-dose QZF group exhibited significantly reduced expressions of P-TH,P-CaMK Ⅱ,and DA-related proteins,alongside decreased mRNA expression of CaMK Ⅱ,TH,and DA.Conclusion QZF significantly alleviates tic,hyperactivity,and attention deficit symptoms in the TD combined with ADHD rat model.Its mechanism may be associated with the regulation of DA-related pathways by CaMK Ⅱ.关键词
强志方/抽动障碍/注意缺陷多动障碍/大鼠模型/分子机制Key words
Qiangzhi Formula(强志方)/Tic disorder/Attention deficit hyperactivity disorder/Rat model/Molecular mechanism分类
医药卫生引用本文复制引用
鲁婷婷,董成达,胡冬青,阎兆君..强志方对抽动障碍共患注意力缺陷多动障碍大鼠的疗效及潜在机制研究[J].时珍国医国药,2025,36(19):3615-3624,10.基金项目
国家自然科学基金(81774249) （81774249）
国家中医药管理局国医大师传承工作室建设项目(国中医药人教发[2018]119号) （国中医药人教发[2018]119号）
山东省中医药管理局齐鲁医派中医学术流派传承项目(鲁卫函[2021]45号 （）
鲁卫函[2022]93号) （）
山东省济南市"高校20条"资助项目(2020GXRC013) （2020GXRC013）
2023年度局省共建中医药科技项目(GZY-KJS-2023-017) （GZY-KJS-2023-017）
