时珍国医国药2025，Vol.36Issue(19)：3632-3638,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1905
皮质酮应激促巨噬细胞M2型极化模型构建及逍遥散体外干预作用的研究
Construction of an M2-type polarized macrophage model induced by corticosterone stress and study on the intervention effect of Xiaoyao Powder(逍遥散)in vitro Powder
摘要
Abstract
Objective To construct an M2-polarized macrophage model induced by corticosterone(CORT)stress and observe the effect of XiaoyaoPowder(逍遥散,XYP).Methods Mouse M2 macrophage model was induced by CORT.CD11c and CD206 of macrophage polarization markers were detected by flow cytometry with different concentrations of CORT.The effects of XYS on the secretion of TGF-β1 and IL-10 in macrophages were detected by ELISA.The effects of XYP on the expression of iNOS and Arg-1 in macrophages were measured by Western blot(WB).Results Phase-contrast microscopy revealed morphological features of M2 polarization in macrophages treated with 200 ng/mL CORT,accompanied by increased expression of the M2 markers CD206(surface protein)and Arg-1,as well as elevated TGF-β1 levels in the supernatant.The IC30 of XYP on RAW264.7 cell viability was determined to be 0.68 mg/mL.CORT sig-nificantly increased the content of TGF-β1 and decreased the content of IL-10 in the supernatant of RAW264.7 cells(P<0.05).The expression of M2 surface protein marker CD206 was the highest in 200ng/mL CORT group,whereas the expression of M1 surface protein marker CD11c was minimal compared to other concentrations(P<0.05).XYP significantly increased the expression of M1-type marker iNOS,while decreased M2 markers CD206 and Arg-1 in CORT-treated macrophages(P<0.05).Conclusion CORT could pro-mote M2-type polarization of macrophages to a certain extent,whereas XYS significantly reversed and regulated the CORT-induced M2 polarization.关键词
巨噬细胞/逍遥散/皮质酮/表型极化/乳腺癌/肿瘤微环境/转化生长因子β1Key words
Macrophages/XiaoyaoPowder(逍遥散)/Corticosterone/Phenotypic polarization/Breast cancer/Tumer microenviroment/TGF-β1分类
医药卫生引用本文复制引用
张文娴,彭梦薇,罗紫龙,匡洛逸,吴耀松,刘燕..皮质酮应激促巨噬细胞M2型极化模型构建及逍遥散体外干预作用的研究[J].时珍国医国药,2025,36(19):3632-3638,7.基金项目
国家自然科学基金(82104717) （82104717）
河南省科技厅河南省重点研发与推广专项(科技攻关)(232102310443) （科技攻关）
河南省科技研发计划联合基金(242301420099) （242301420099）
河南省科技攻关计划项目(222102310649) （222102310649）
