时珍国医国药2025，Vol.36Issue(19)：3646-3654,9.DOI:10.70976/j.1008-0805.SZGYGY-2025-1907
痛泻要方对葡聚糖硫酸钠诱导溃疡性结肠炎小鼠肠道菌群及短链脂肪酸的影响
Effects of Tongxie Yaofang(痛泻要方)on intestinal microbiota and short-chain fatty acids in DSS-induced mice with ulcerative colitis
摘要
Abstract
Objective To investigate the effects of Tongxie Yaofang(痛泻要方,TXYF)on intestinal microbiota and the metabolite short-chain fatty acids(SCFAs)in dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)mice.Methods 27 SPF-grade male Balb/c mice were randomly divided into groups.The model and treatment groups received 5%(w/v)DSS in drinking water to induce UC.The treatment group was administered TXYF(11.70 g/kg)via oral gavage for 7 days.Post-intervention,serum levels of IL-10,IL-6,TNF-α,IL-1β,and TGF-β were measured using ELISA.Ileal contents were subjected to 16S rRNA gene sequencing and SCFAs quantification.Results TXYF promoted mucosal structural recovery,alleviated inflammatory infiltration,increased serum IL-10 levels,and reduced serum IL-6 and TNF-α levels(P<0.05).Sequencing identified 407 shared operational taxonomic units(OTUs).TXYF intervention elevated the Chao1 and Faith's phylogenetic diversity(PD)indexes,significantly altered Beta diversity(P<0.01),increased the relative abundance of the Verrucomicrobia and TM7 phyla,and the Firmicutes/Bacteroidetes ratio(P<0.05).Further-more,18 bacterial taxa showed differential abundance compared to controls.At the genus level,serum TGF-β1 levels were significantly correlated with the relative abundance of Desulfovibrio and Sulfuricurvum(P<0.01),while serum IL-6 levels were significantly corre-lated with the relative abundance of Subdoligranulum and Coprococcus(P<0.01).At the phylum level,serum TNF-α levels were cor-related with the relative abundance of Verrucomicrobia(P<0.05),and serum IL-1β levels were correlated with the relative abundance of TM7,TM6,and OD1(P<0.05).SCFAs quantification indicated TXYF elevated acetic and butyric acid concentrations while reduc-ing propionic acid(P<0.05).Conclusion TXYF alleviated mucosal barrier damage in colitis models by reducing pro-inflammatory cytokines(IL-6,TNF-α),elevating levels of acetic acid and butyric acid,and lowering the level of propionic acid,highlighting its association with modulation of gut microbiota and SCFAs metabolism.关键词
痛泻要方/溃疡性结肠炎肠道菌群/短链脂肪酸/代谢组学/16S rRNA/气相色谱质谱法Key words
Tongxie Yaofang(痛泻要方)/Ulcerative colitis/Gut microbiota/Short-chain fatty acids/Metabolomics/16S rRNA/GC-MS分类
医药卫生引用本文复制引用
郑灿磊,于斌,厉启芳,颜晓,梁凤霞..痛泻要方对葡聚糖硫酸钠诱导溃疡性结肠炎小鼠肠道菌群及短链脂肪酸的影响[J].时珍国医国药,2025,36(19):3646-3654,9.基金项目
国家自然科学基金(81603509) （81603509）
山东省自然科学基金资助项目(ZR2014HQ051) （ZR2014HQ051）
济宁市重点研发计划项目(2021YXNS007) （2021YXNS007）
