时珍国医国药2025，Vol.36Issue(19)：3655-3660,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-1908
基于GLUT4-mTOR信号通路探讨金锁固精丸加味方对糖尿病肾病大鼠的影响
Exploring the effects of Modified Jinsuo Gujing Pill(金锁固精丸加味方)on rats with diabetic nephropathy based on GLUT4-mTOR signaling pathway
摘要
Abstract
Objective To investigate the mechanism of Modified Jinsuo Gujing Pill(金锁固精丸加味方,MJGP)in ameliorating dia-betic nephropathy(DN)in rats.Methods Eighty-four male SD rats were divided into a normal control group(n=8)and a modeling group(n=76).The modeling group was induced with a high sugar and high-fat diet(HFD)combined with streptozotocin.Upon success-ful induction,the modeling group was subdivided into four groups:the model group,low-dose MJGP group(12.78 g/kg),high-dose MJGP group(19.17 g/kg)of Chinese herbal medicine(CHM),and rapamycin group(0.05 mg/kg).After 4 weeks of treatment,three rats from each group(including the normal control group)were randomly selected.The protein expression levels in glomeruli were ana-lyzed by Western blot(WB),which included GLUT4 involved in glucose transport and metabolic regulation,mTOR and its activated form p-mTOR in the key pathways of cell growth and metabolism,nephrin,podocin and α-actinin-4 related to the structural stability of podocytes.In addition,it also includes Insr,which regulates upstream of the mTOR pathway,the components of the mTOR complex rictor and raptor,as well as their downstream effector proteins p70 S6 Kinase and p-p70 S6 Kinase.Results Compared with the normal control group,the protein expressions of GLUT4,nephrin,podocin,Insr,p70 S6 Kinase,and p-p70 S6 Kinase in the model rats were significantly reduced(P<0.05),while the protein expressions of mTOR,p-mTOR,raptor,rictor,and α-actinin-4 were significantly increased(P<0.05).Compared with the model group,the high-dose group of CHM and the rapamycin group showed significantly increased expressions of GLUT4,nephrin,p70 S6 Kinase,p-p70 S6 Kinase,and Insr proteins(P<0.05),while the expressions of mTOR,p-mTOR,raptor,alpha-actinin-4,and rictor proteins were significantly inhibited(P<0.05).Conclusion MJGP may repair podocyte injury by regulating GLUT4-mTOR signaling pathway and delay the progression of DN in rats.关键词
糖尿病肾病/金锁固精丸加味方/雷帕霉素/足细胞/葡萄糖转运蛋白4Key words
Diabetic nephropathy/Modified Jinsuo Gujing Pill(金锁固精丸加味方)/Rapamycin/Podocyte/Glucose trans-porter4(GLUT4)分类
医药卫生引用本文复制引用
李秀滢,张奡,林惠婷,刘柳,张秋林..基于GLUT4-mTOR信号通路探讨金锁固精丸加味方对糖尿病肾病大鼠的影响[J].时珍国医国药,2025,36(19):3655-3660,6.基金项目
广东省中医药局重点项目(20193003 （）
20241016) （）
