山西中医药大学学报2025，Vol.26Issue(8)：855-860,6.DOI:10.19763/j.cnki.2096-7403.2025.08.05
黄芪多糖对罗丹明123在大鼠体内口服吸收的影响
Effect of astragalus polysaccharides on the oral absorption of Rhodamine 123 in rats
摘要
Abstract
Objective:To validate whether astragalus polysaccharides(APS)inhibit P-glycoprotein(P-gp)function in rat intestinal epithelium by evaluating their effects on oral absorption of Rhodamine 123(Rh123),a prototypical P-gp substrate.Methods:APS was extracted via water extraction-alcohol precipitation.APS and protein content in Huangqi were quantified using phenol-sulfuric acid method and BCA protein assay kit,respectively.Thirty SD rats were divided into:blank control(purified water),positive control(0.01 mg/g verapamil),and APS groups(0.1 mg/g)with different adminis-tration durations(2 d,5 d,10 d;n=6/group).Each administration group received intragastic administration once daily at a dosage of 1 mg/100 g.Thirty minutes after the last administration,rats were orally administered Rh123(0.005 mg/g,1 ml/100 g).Plasma Rh123 concentrations were measured via fluorescence microplate reader at 15,30,60,90,120,180,240,and 360 min post-dosing.Pharmacokinetic parameters were calculated using Phoenix WinNonlin software.Results:APS con-tent was 54.09%±1.69%and protein content 8.89%±0.30%.Compared with the blank group:APS-2 d group showed sig-nificantly increased AUC0-t(P<0.01);APS-5 d and verapamil groups exhibited significantly increased AUC0-tand Cmax(P<0.01);APS-10 d group showed no significant pharmacokinetic differences(P>0.05).No significant differences in Cmax or AUC0-t were observed between APS-2 d/5 d and verapamil groups(P>0.05),while APS-10 d showed significantly lower values than verapamil(P<0.01).Conclusion:APS enhances oral absorption of Rh123 in rats,indicating P-gp inhibition in intesti-nal epithelial cells,with maximal effect at 5-day administration.关键词
黄芪多糖/P-糖蛋白/罗丹明123/药代动力学Key words
astragalus polysaccharides/P-glycoprotein/Rhodamine 123/pharmacokinetics分类
医药卫生引用本文复制引用
余晓夏,蒋畅,雷婷,陈念,张捷,韩静..黄芪多糖对罗丹明123在大鼠体内口服吸收的影响[J].山西中医药大学学报,2025,26(8):855-860,6.基金项目
福建省属公益类科研院所基本科研专项项目(2024R1003009) （2024R1003009）
