山西中医药大学学报2025，Vol.26Issue(8)：861-866,872,7.DOI:10.19763/j.cnki.2096-7403.2025.08.06
基于子午流注择时中药保留灌肠对脓毒症胃肠功能障碍大鼠炎症因子、肠道菌群及TRPV1表达的研究
Study on the effects of meridian timing-based Chinese herbal retention enema on inflammatory factors,gut microbiota,and trpv1 expression in septic rats with gastrointestinal dysfunction
摘要
Abstract
Objective:To investigate the effects of meridian timing-based Chinese herbal retention enema on serum in-flammatory factors,gut microbiota,and expression of high mobility group box 1(HMGB1),calcitonin gene-related pep-tide(CGRP),and transient receptor potential vanilloid 1(TRPV1)proteins in small intestine tissue of septic rats with gas-trointestinal dysfunction.Methods:Forty-eight rats were randomly assigned to sham group(n=8),model group(n=10),and enema groups 1/2/3(n=10 each).Sepsis-induced gastrointestinal dysfunction models were established in all groups except sham group.Enema groups received Major Purgative Decoction(Dachengqi Tang,4.32 g crude herb/kg,1 ml/rat)retention enema at designated times:Group 1(You period,6:00 PM),Group 2(Chen period,9:00 AM),Group 3(Mao period,6:00 AM).Sham and model groups received saline enema at Mao period.All groups received enema once daily for 14 con-secutive days.Serum TNF-α,IL-1β,IL-6 were measured by ELISA;gut microbiota(Bifidobacterium,Lactobacillus)analyzed via high-throughput sequencing;HMGB1 expression detected by immunohistochemistry;TRPV1 and CGRP ex-pression by Western blot;intestinal morphology assessed via HE staining.Results:Compared with sham group,model group showed elevated serum inflammatory factors,HMGB1/TRPV1/CGRP expression,and Bifidobacterium abundance with re-duced Lactobacillus abundance(P<0.05).All enema groups exhibited reduced inflammatory factors,HMGB1/TRPV1/CGRP expression,Bifidobacterium abundance and increased Lactobacillus abundance versus model group(P<0.05).Among enema groups,Group 3 demonstrated the lowest inflammatory markers,HMGB1/TRPV1/CGRP expression,Bifidobacterium abun-dance and highest Lactobacillus abundance(P<0.05 vs Groups 1/2).No significant differences existed between Groups 1 and 2(P>0.05).Model group showed intestinal villi epithelial detachment,inflammatory infiltration,and disordered gland ar-rangement;all enema groups exhibited improved morphology with reduced inflammation,most significantly in Group 3.Conclusion:Meridian timing-based herbal enema effectively reduces inflammatory factors,modulates gut microbiota bal-ance,downregulates HMGB1/TRPV1/CGRP expression,and improves intestinal morphology in septic rats with gastrointesti-nal dysfunction.关键词
脓毒症/胃肠功能障碍/子午流注择时/中药保留灌肠/肠道菌群Key words
sepsis/gastrointestinal dysfunction/meridian timing/Chinese herbal retention enema/gut microbiota分类
医药卫生引用本文复制引用
杨海玲,毛艳,方蓉,张晓琳..基于子午流注择时中药保留灌肠对脓毒症胃肠功能障碍大鼠炎症因子、肠道菌群及TRPV1表达的研究[J].山西中医药大学学报,2025,26(8):861-866,872,7.基金项目
云南省科技厅科技计划项目(202101AZ070001-091) （202101AZ070001-091）
