山西中医药大学学报2025，Vol.26Issue(8)：889-897,9.DOI:10.19763/j.cnki.2096-7403.2025.08.10
基于网络药理学探讨国医大师许润三教授"加味消瘰丸"治疗子宫肌瘤的作用机制
Exploring the mechanism of Master Xu Runsan's Supplemented Scrofula-Removing Pill in treating uterine fibroids based on network pharmacology
摘要
Abstract
Objective:To investigate the mechanism of Master Xu Runsan's self-formulated Supplemented Scrofula-Removing Pill(Jiawei Xiaolei Wan)in treating uterine fibroids(UF)using network pharmacology.Methods:Active ingre-dients of the herbs in Supplemented Scrofula-Removing Pill and their potential targets were collected from the TCMSP and BATMAN databases,while UF-related disease targets were retrieved from GeneCards,OMIM,and DisGeNET databases.A"herb-active ingredient-disease target"network was constructed using Cytoscape 3.10.0.The protein-protein interaction(PPI)network for Supplemented Scrofula-Removing Pill against UF was built via the STRING database.Core therapeutic targets were screened based on topological parameters(degree value).Functional enrichment analysis of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways was performed on intersecting targets using the DAVID database.Results:Through screening,87 active ingredients were identified,with quercetin,luteolin,kaemp-ferol,wogonin,and crocetin exhibiting the highest target associations.Venny 2.1.0 analysis revealed 193 predicted targets for UF treatment;topological analysis identified p53,Akt1,ESR1,TNF,JUN,IL6,HSP90AA1,EGFR,CCND1,and MAPK1 as core targets(ranked by degree value).GO functional enrichment yielded 957 entries:biological processes(BP,717 entries)involved positive regulation of gene expression,RNA polymerase Ⅱ promoter transcription,response to exogenous stimuli,and DNA-templated transcription;molecular functions(MF,159 entries)focused on enzyme binding,protein binding,and transcription factor activity;cellular components(CC,81 entries)were enriched in extracellular space and chromatin.KEGG pathway enrichment identified 178 pathways,primarily including cancer pathways,AGE-RAGE signaling in diabetic complications,fluid shear stress and atherosclerosis,and the PI3K-Akt signaling pathway.Conclusion:This study provides a theoretical foundation for developing traditional Chinese medicine(TCM)compound formulas and offers critical insights for further mechanistic research on Supplemented Scrofula-Removing Pill in UF treat-ment.关键词
子宫肌瘤/加味消瘰丸/中药复方/网络病理学/作用机制Key words
uterine fibroids/Supplemented Scrofula-Removing Pill/TCM compound/network pharmacology/mechanism of action分类
医药卫生引用本文复制引用
杨舫,王清,刘弘,赵芳,梁静..基于网络药理学探讨国医大师许润三教授"加味消瘰丸"治疗子宫肌瘤的作用机制[J].山西中医药大学学报,2025,26(8):889-897,9.基金项目
中央高水平医院临床研究和成果转化能力试点项目(2022-NHLHCRF-LX-02-0116) （2022-NHLHCRF-LX-02-0116）
北京中医药薪火传承"新3+3"工程许润三"三名"传承工作室(2023-SZ-F-07) （2023-SZ-F-07）
