生物骨科材料与临床研究2025，Vol.22Issue(5)：1-8,16,9.DOI:10.3969/j.issn.1672-5972.2025.05.001
DLD基因通过PI3K/AKT通路对激素性股骨头坏死的BMSCs的影响
The impact of the DLD gene on BMSCs in steroid-induced femoral head necrosis via the PI3K/AKT pathway
摘要
Abstract
Objective To analyze the effect of DLD gene on the cell model of steroid-induced femoral head necrosis(SNFH)using bone marrow mesenchymal stem cells(BMSCs)via the PI3K/AKT signaling pathway.Methods BMSCs were cultured and divided into four groups:BMSCs,Model,Model+si-NC,and Model+si-DLD.The expression of DLD was analyzed using polymerase chain reaction(PCR)and Western Blot(WB).Cell proliferation and apoptosis were assessed using CCK-8 and TUNEL assays,respectively.Oxidative stress levels were determined by measuring reactive oxygen species(ROS).PCR and WB were also employed to evaluate markers of osteogenesis(RUNX2),adipogenesis(PPAR-γ),apoptosis(Bax/Bcl-2),angiogenesis(VEGF),oxidative stress(HO-1),and PI3K/AKT pathway activity.Bone formation and adipogenic differentiation were analyzed using Alizarin Red and Oil Red O staining.Results Overexpression of DLD decreased in the levels of p-PI3K and p-AKT,thereby inhibiting the PI3K/AKT pathway.This results in reduced calcium deposition,increased adipocyte formation,elevated apoptosis,decreased angiogenesis,enhanced oxidative stress,and impaired cell proliferation.Conclusion The DLD gene inhibits the activation of the PI3K/AKT signaling pathway,thereby suppressing osteogenic differentiation and promoting adipogenic differentiation in the BMSCs cell model of SNFH.关键词
激素性股骨头坏死/二氢硫辛酰胺脱氢酶/PI3K/AKT通路/骨髓间充质干细胞Key words
Steroid-induced femoral head necrosis/DLD/PI3K/AKT pathway/Bone marrow mesenchymal stem cells分类
医药卫生引用本文复制引用
卢兴宝,霍嘉琪,原天祎,刘洪江,马在超,麦麦提依不巴吉,杨增强,罗超,崔泳..DLD基因通过PI3K/AKT通路对激素性股骨头坏死的BMSCs的影响[J].生物骨科材料与临床研究,2025,22(5):1-8,16,9.基金项目
国家自然科学基金(81960396) （81960396）
