摘要
Abstract
Objective To investigate the protective effects of Lupenone on chondrocyte inflammation and osteoarthritis(OA)in mice.Methods Chondrocytes were obtained from the knee joints of C57BL/6 mice and treated with various concentrations of Lupenone(0-100 µM).Cell viability following IL-1β stimulation was assessed using the CCK-8 assay.The chondrocytes were subsequently categorized into a control group,an IL-1β group,and an IL-1β+Lupenone group.IL-6 and TNF-α levels were measured using ELISA,while the gene expressions of MMP-3,MMP-13,and ADAMTS5 were quantified via RT-qPCR.A mouse osteoarthritis(OA)model was constructed,and 8-week-old male mice were randomly allocated to sham operation group,DMM group,and DMM+Lupenone treatment group.The DMM+Lupenone group received intraperitoneal injections of Lupenone(8 mg/kg)twice daily post-surgery,while the other groups received equivalent volumes of PBS.Joint damage was evaluated through gait analysis and histological staining(Hematoxylin-Eosin and Safranin O-Fast green staining),with ADAMTS5 expression assessed using OARSI scoring and immunohistochemistry.Results Lupenone at 100 μM significantly reduced chondrocyte viability(P<0.05),while 20 μM effectively inhibited the IL-1β-induced decline in viability.Additionally,Lupenone markedly reduced IL-6 and TNF-α levels,as well as the gene expressions of MMP-3,MMP-13,and ADAMTS5 in IL-1β-stimulated chondrocytes.In OA mice,the DMM group exhibited substantial joint damage,with increased OARSI scores and elevated ADAMTS5 expression.In contrast,the DMM+Lupenone group demonstrated improved gait,attenuated joint damage,and significantly lower OARSI scores and ADAMTS5 expression(P<0.05).Conclusion Lupenone inhibits IL-1 β-induced chondrocyte inflammation and extracellular matrix degradation while mitigating joint damage and gait abnormalities in OA mice.关键词
羽扇豆酮/骨关节炎/软骨细胞/炎症反应/小鼠Key words
Lupenone/Osteoarthritis/Chondrocyte/Inflammation/Mouse分类
医药卫生
