畜牧兽医学报2025，Vol.56Issue(9)：4315-4327,13.DOI:10.11843/j.issn.0366-6964.2025.09.016
吐鲁番黑羊体重和体尺性状全基因组关联分析
Genome-Wide Association Study of Body Weight and Body Size Traits In Turpan Black Sheep
摘要
Abstract
This study aimed to identify candidate genes and molecular markers associated with body weight and body size traits in Turpan black sheep using genome-wide association study(GWAS),thereby providing a scientific basis for genetic breeding and resource development of Turpan black sheep.In this study,129 healthy male Turpan black sheep aged 1 to 3 years,raised under the uniform conditions,were selected.Phenotypic data for 9 traits were measured:body weight,body height,body length,chest circumference,tube circumference,chest width,chest depth,tail width,and tail length.Peripheral blood samples(5 mL)were collected from the jugular vein using EDTA-K2 anticoagulant vacuum tubes for DNA extraction.Qualified DNA samples underwent genotyping-by-sequencing(GBS).Raw sequencing data were processed,filtered,and annotated using software such as samtools and bcftools.Subsequently,population genetic structure analysis,population stratification assessment,and GWAS were performed using GCTA,TreeBeST,and GEMMA softwares,respectively.Sequencing yielded 160.88 G of raw data.After quality control,157.87 G of clean data were obtained,identifying 460 766 SNP loci.GWAS detected 74 significant SNPs(P＜10-5)associated with the 9 traits.Annotation revealed 39 candidate genes.Haplotype analysis showed that multiple significant SNPs were located within haplotype blocks.This study identified important genetic loci and candidate genes associated with body weight and body size traits of Turpan black sheep.The findings provide crucial insights for elucidating the genetic mechanisms underlying these traits and lay the foundation for molecular marker-assisted breeding in Turpan black sheep.关键词
吐鲁番黑羊/体尺性状/全基因组关联分析/单倍型分析Key words
Turpan black sheep/body size traits/genome-wide association study/haplotype analysis分类
农业科技引用本文复制引用
白锋,玛尔孜娅·亚森,阿米妮古丽·阿不来孜,滕文,罗春彦,纳扎开提·艾尼万尔,张耘韬,纪新民,张艳花..吐鲁番黑羊体重和体尺性状全基因组关联分析[J].畜牧兽医学报,2025,56(9):4315-4327,13.基金项目
中央引导地方科技发展专项资金:羊遗传资源评价与利用实验平台建设(ZYYD2023B10) （ZYYD2023B10）
新疆自治区产业技术体系(XJARS-09-05) （XJARS-09-05）
吐鲁番市重点研发专项:吐鲁番黑羊核心群选育体系建立及优质肉产品开发(202316) （202316）
新疆牧区地方肉羊选育改良计划(XJMQ-1) （XJMQ-1）
