西北农业学报2025，Vol.34Issue(9)：1573-1582,10.DOI:10.7606/j.issn.1004-1389.2025.09.001
虹鳟肝脏响应运输胁迫的转录组学分析
Transcriptomic Analysis of Rainbow Trout Liver Response to Transport Stress
摘要
Abstract
To explore the physiological mechanisms of rainbow trout in response to transportation stress,a transport stress test in this study was conducted by simulating live fish transport.Liver tis-sue samples were collected from the control group(transported for 0 h)and the treatment group(transported for 8 h)for transcriptome analysis.A total of 4 389 differentially expressed genes(DEGs)were identified between the two groups,of which 2 526 DEGs were upregulated and 1 863 DEGs were downregulated.Gene Ontology(GO)enrichment analysis revealed that DEGs were main-ly associated with transferase activity and catalytic activity,and were significantly enriched in cell me-tabolism processes and intracellular receptor signaling pathways.KEGG enrichment analysis indicated that DEGs were significantly enriched in pathways related to energy metabolism and immune respon-ses.The expression levels of phosphoenolpyruvate carboxykinase(pepck)and glucose-6-phosphatase(g6pc)in the insulin signaling pathway;interleukin-3(il-3)and the anti-apoptosis gene BCL2-like 1(bcl-xl)in the apoptosis pathway;and B-cell lymphoma 6(bcl-6)in the FoxO signaling pathway were significantly upregulated after 8 hours of transport stress,while cholestenol Delta-isomerase(ebp),squalene epoxidase(sqle),Delta24-sterol reductase(dhcr24),and farnesyl-diphosphate farne-syltransferase 1(fdft1)were significantly downregulated.In addition,the transcriptome results were validated by qRT-PCR analysis of seven candidate genes,which confirmed the expression trends ob-served in the transcriptome data.关键词
虹鳟/转录组/运输胁迫/糖脂代谢/细胞凋亡Key words
Rainbow trout/Transcriptome/Transport stress/Glycolipid metabolism/Apoptosis引用本文复制引用
刘绩昌,康玉军,权金强,朱利瑞,宋国林,刘哲..虹鳟肝脏响应运输胁迫的转录组学分析[J].西北农业学报,2025,34(9):1573-1582,10.基金项目
国家自然科学基金(32260923) （32260923）
甘肃省现代丝路寒旱农业科技支撑计划(GSLK-2022-11). The National Natural Science Foundation of China(No.32260923) （GSLK-2022-11）
Modern Silk Road Cold and Drought Agricultural Science and Technology Support Project of Gansu Province(No.GSLK-2022-11). （No.GSLK-2022-11）
