西北农业学报2025，Vol.34Issue(9)：1665-1676,12.DOI:10.7606/j.issn.1004-1389.2025.09.010
荷花(Nelumbo nucifera)MYB6-like转录因子的克隆及其功能验证
Cloning and Functional Validation of MYB6-like Transcription Factor in Nelumbo nucifera
摘要
Abstract
The unique aquatic environment oflotus(Nelumbo nucifera)brings numerous biological and abiotic stresses to its growth and development.Proanthocyanidins serve as a crucial defense mech-anism for plants against these stresses.However,the molecular mechanism underlying their synthe-sis in lotus remains unclear.We screened and cloned NnMYB6-like gene related to proanthocyanidin synthesis in lotus,performed bioinformatics analysis,and conducted transient overexpression in'Taikonglian'to analyze its function in the proanthocyanidin biosynthesis pathway by RNA-Seq.The results showed that transient overexpression of NnMYB6-like gene in'Taikonglian'lotus leaves sig-nificantly up-regulated the expression of key genes involved in the anthocyanin synthesis pathway,in-cluding NnANS,NnF3H and NnLAR,compared to the wild-type.Notably,NnLAR played a cruci-al role in proanthocyanidin metabolism.Moreover,the proanthocyanidin content was substantially in-creased in lotus leaves with transient overexpression.The transiently overexpressed lotus displayed a significantly higher proanthocyanidin content compared to the wild-type,suggesting that the NnMYB6-like gene may exert positive regulation on the proanthocyanidin synthesis pathway in lotus.The results provide a theoretical foundation for the exploration of the molecular mechanism of proan-thocyanidin synthesis in lotus.关键词
荷花/MYB转录因子/原花青素/瞬时过表达/生物信息学Key words
Nelumbo nucifera/MYB transcription factor/Proanthocyanidin/Transient overexpres-sion/Bioinformatics引用本文复制引用
蔡月琴,赵琬玥,林玉雪,熊瑜,钟建辉,陈龙清,谯正林..荷花(Nelumbo nucifera)MYB6-like转录因子的克隆及其功能验证[J].西北农业学报,2025,34(9):1665-1676,12.基金项目
云南省重大科技专项计划(202202AE090028) （202202AE090028）
云南省高层次人才引进计划高端外国专家专项(YNQR-GDWG-2020-001). Special Program of Major Science and Technology of Yunnan Province(No.202202AE090028) （YNQR-GDWG-2020-001）
High-End Foreign Experts Program,the High-Level Talent Recruitment Plan of Yunnan Province(No.YNQR-GDWG-2020-001). （No.YNQR-GDWG-2020-001）
