黄芪查尔酮异构酶的全基因组鉴定及分析
Genome-wide Identification and Analysis of Chalcone Isomerase Gene Family in Astragalus membranaceus
摘要
Abstract
To elucidate the functional roles of chalcone isomerase(CHI)genes in Astragalus mem-branaceus,we performed a comprehensive genome-wide identification and analysis of the CHI gene.A total of ten AmCHI genes were identified through integrated genomic and bioinformatics approaches.Subsequent analyses of their physicochemical properties,phylogenetic relationships,conserved motifs,and promoter cis-acting elements revealed key insights into their structural and functional features.The AmCHI genes are unevenly distributed across chromosomes,and their expansion appears to be predominantly driven by segmental and tandem duplication events under purifying selection pressure.Phylogenetic analysis indicated a high degree of evolutionary conservation between AmCHI proteins and CHI homologs from other plant species.Promoter element analysis suggested that AmCHI gene expression is likely regulated by abscisic acid,methyl jasmonate,photoperiod,and various abiotic stres-ses.Furthermore,qRT-PCR results analysis demonstrated tissue-specific expression patterns,with no-tably high transcript levels observed in leaves.关键词
黄芪/CHI基因家族/基因复制/系统进化/基因表达Key words
Astragalus membranaceus/CHI gene family/Gene duplication/Phylogenetic/Gene ex-pression引用本文复制引用
李怀珠,窦玲玲,张博文,韩佳乐..黄芪查尔酮异构酶的全基因组鉴定及分析[J].西北农业学报,2025,34(9):1685-1693,9.基金项目
陕西省教育厅专项科研计划(22JK0602) （22JK0602）
陕西省大学生创新创业训练计划(S202310722092) （S202310722092）
陕西省自然科学基础研究计划(2022JQ-190) （2022JQ-190）
陕西省教育厅高校工程研究中心项目(24JR162) （24JR162）
黄土高原道地药材品种选育与生态栽培科技创新团队项目(2022) （2022）
黄土高原道地药材陕西省高校工程研究中心项目(2023). Special Research Program of Shaanxi Provincial Education Department(No.22JK0602) （2023）
Shaanxi College Students Innovation and Entrepreneurship Training Program(No.S202310722092) （No.S202310722092）
Shaanxi Provincial Natural Science Basic Research Plan Project(No.2022JQ-190) （No.2022JQ-190）
Project of the Shaanxi Provincial Education Departments's University Engineering Research Centers(No.24JR162) （No.24JR162）
The Youth Innovation Team of Universities in Shaanxi Province(No.2022) （No.2022）
Engi-neering Research Center of Loess Plateau Authentic Medicinal Herbs for Universities in Shaanxi Prov-ince(No.2023). （No.2023）
