烟台大学学报（自然科学与工程版）2025，Vol.38Issue(4)：412-418,7.DOI:10.13951/j.cnki.37-1213/n.240509
基于微卫星标记评估回捕阴山湾条石鲷增殖放流效果
Evaluation of Stock Enhancement Effect of Oplegnathus fasciatus in Yinshan Bay Based on Microsatellite Markers
摘要
Abstract
Five pairs of microsatellite primers were used to identify the paternity of Oplegnathus fasciatus in Yinshan Bay,Weihai,Shandong Province,to evaluate the stock enhancement of O.fasciatus in June 2023.The results of the microsatellite polymorphism analysis showed that the average number of alleles(Na),average observed het-erozygosity(Ho),average expected heterozygosity(He),and average polymorphism information content(PIC)for 158 breeding parents and 542 recaptured individuals were 33.6,0.709 2,0.829 6,and 0.819,respectively.The results of the parent-child relationship analysis showed that the non-parental exclusion rates(NEP)and cumu-lative non-parental exclusion rates(CEP)for the five microsatellite loci ranged from 0.191 to 0.836 and 0.994 296 65,respectively,when the gender of the parents was unknown.Therefore,the comprehensive analysis showed that among the 542 recaptured individuals,8 were confirmed as offspring of the parents from this stock enhancement in the simulation analysis,and the contribution rate of this stock enhancement to recaptured individu-als was 1.48％.关键词
条石鲷/分子标记/增殖放流/效果评价/阴山湾海域Key words
Oplegnathus fasciatus/molecular marker/stock enhancement/effect evaluation/Yinshan Bay分类
农业科技引用本文复制引用
丁悦,任中杰,徐鑫,吕廷晋,娄方瑞..基于微卫星标记评估回捕阴山湾条石鲷增殖放流效果[J].烟台大学学报（自然科学与工程版）,2025,38(4):412-418,7.基金项目
山东省自然科学基金资助项目(ZR2021QD110) （ZR2021QD110）
烟台大学横向科研项目(HX23KH050). （HX23KH050）
