医药导报2025，Vol.44Issue(10)：1525-1530,6.DOI:10.3870/j.issn.1004-0781.2025.10.001
维生素D对MRL/lpr狼疮小鼠Tfh细胞的影响及其机制
Effect and Mechanism of Vitamin D on Tfh Cells in MRL/lpr Lupus Mice
摘要
Abstract
Objective To study the effect and molecular mechanism of vitamin D(VitD)on Tfh cells of MRL/lpr lupus mice.Methods C57/B6 mice and MRL/lpr lupus mice were transfected with siRNA to construct VDR knockout mouse models.Splenic Tfh cells of C57/B6 mice and MRL/lpr lupus mice were divided into control group,lupus group and VDRsiRNA lupus group(treated with vitamin D 0,1 and 10 nmol·L-1,respectively)by siRNA transfection.The percentage of Tfh cells was detected by flow cytometry.MRL/lpr lupus mice Peyer node Tfh cells were randomly divided into 7 groups,blank control group,vitamin D dose groups of 1 and 10 nmol·L-1,paricalcitol group(VitD 10 nmol·L-1+PA),VDRsiRNA control group,VDRsiRNA group(VitD 10 nmol·L-1),CaN inhibitor group(VitD 10 nmol·L-1+CsA),and incubated for 72 h.The concentration of calcium ions in Tfh of each group was detected.The expressions of AT1R,NFAT,CaN and P-CaN in Tfh cells were determined by Western blotting.Results The percentage of Tfh cells decreased significantly with the increase of vitamin D dose.Vitamin D CaN reduce the intracellular calcium concentration of Tfh,up-regulate the expression of AT1 protein in Tfh cells,and down-regulate the expression of CAN,P-CaN and NFAT protein in a dose-dependent manner,and the effect is more obvious when combined with PA.Conclusion Vitamin D may regulate the activation of follicular T helper cells in MRL/lpr mice via the Ca-CaN-NFAT pathway.关键词
维生素D/系统性红斑狼疮/滤泡辅助性T细胞/Ca-CaN-NFATKey words
Vitamin D/Systemic lupus erythematosus/Follicular helper T cells/Ca-CaN-NFAT分类
医药卫生引用本文复制引用
侯晓婷,李琳,袁方,邵翠,尹太勇,丁冬胜,徐洪涛,邹敏书..维生素D对MRL/lpr狼疮小鼠Tfh细胞的影响及其机制[J].医药导报,2025,44(10):1525-1530,6.基金项目
国家自然科学基金资助项目(81373179). （81373179）
