医药导报2025，Vol.44Issue(10)：1531-1540,10.DOI:10.3870/j.issn.1004-0781.2025.10.002
基于转录组学探讨葛根芩连汤通过干预HepG2细胞改善非酒精性脂肪性肝病的作用机制
Explore the Mechanism of Gegen Qinlian Decoction in Ameliorating Nonalcoholic Fatty Liver Disease Through Effect HepG2 Cells Based on Transcriptomics
摘要
Abstract
Objective To explore the potential mechanism of Gegen qinlian decoction(GGQLD)containing serum in ameliorating nonalcoholic fatty liver disease(NAFLD)in human hepatocellular carcinoma HepG2 cells based on transcriptomics.Methods An in vitro model of NAFLD was constructed by free fatty acid(FFA)-induced fat accumulation in HepG2 cells,and cells were treated with different proportions of GGQLD and pioglitazone-containing serum.The lipid deposition in each group was detected by oil red O staining,and the lipid content in each group was evaluated by triglyceride level.Transcriptome technology was used to detect the differentially expressed genes between the intervention groups,and GO annotation analysis,KEGG enrichment analysis and protein interaction(PPI)network analysis were performed to verify the differentially expressed genes by RT-PCR.Results Compared with normal control group,the number of red lipid droplets in the model control group increased,and the triglyceride content increased significantly(P＜0.01).Compared with model control group,the content of red lipid droplets in the GGQLD medium dose group showed a decreasing trend,and the intracellular triglyceride content decreased significantly(P＜0.05).A total of 608 differentially expressed genes were identified by transcriptome analysis,of which 163 differentially expressed genes were up-regulated and 445 differentially expressed genes were down-regulated.GO enrichment analysis showed that the differentially expressed genes were mainly involved in the regulation of MAP kinase phosphatase activity.KEGG analysis showed that the differentially expressed genes were mainly involved in MAPK signaling pathway.RT-PCR results showed that GGQLD up-regulated the expression level of MAP2K6 mRNA and down-regulated the expression levels of FOSL1,CTSL,DUSP5,DUSP1,JUN,HSPA6,IL1A,IL11 and RELB mRNA,which may be mainly involved in MAPK signaling pathway.Conclusion GGQLD has the effect of improving NAFLD,which may be related to MAPK signaling pathway.关键词
葛根芩连汤/转录组学/非酒精性脂肪性肝病/HepG2 细胞Key words
Gegen qinlian decoction/Transcriptomics/Nonalcoholic fatty liver disease/HepG2 cells分类
医药卫生引用本文复制引用
吴爱兰,操映倩,谢佩瑶,曹征,彭淑红,程子文,曹岚,章常华,梁芳..基于转录组学探讨葛根芩连汤通过干预HepG2细胞改善非酒精性脂肪性肝病的作用机制[J].医药导报,2025,44(10):1531-1540,10.基金项目
赣鄱俊才-江西省主要学科学术和技术带头人培养项目(领军人才-学术类)(20232BCJ22022) （领军人才-学术类）
国家自然科学基金资助项目(82060741) （82060741）
江西省教育厅重点项目(GJJ213111) （GJJ213111）
江西省中药药理重点实验室(2024SY07111). （2024SY07111）
