现代中西医结合杂志2025，Vol.34Issue(15)：2065-2069,2088,6.DOI:10.3969/j.issn.1008-8849.2025.15.004
黄芪多糖对阿尔茨海默病小鼠认知功能及小胶质细胞介导神经炎症的影响
Astragalus polysaccharide improves cognitive impairment in Alzheimer's disease mice via inhibiting microglial activation and neuroinflammation
摘要
Abstract
Objective It is to explore the neuroprotective mechanism of Astragalus polysaccharides(APS)in Alzhei-mer's disease(AD)mice and its effects on cognitive function based on microglia-mediated neuroinflammation.Methods Ten wild-type mice were taken as the wild-type group.Additionally,20 transgenic mice carrying 5 familial Alzheimer's dis-ease gene mutations(5×FAD)were taken and randomly divided into 5×FAD transgenic group and 5×FAD transgenic+APS group,with 10 mice in each group.The mice of the 5×FAD transgenic+APS group was given APS 200 mg/(kg·d)by gavage,the mice of the wild-type group and 5×FAD transgenic group were given equal volumes of normal saline by ga-vage,all groups were continuously treated for 60 days.The spatial learning and memory abilities of the mice were assessed by Morris water maze,the morphological changes in hippocampal Iba+microglia were observed by immunohistochemistry,and the mRNA expressions of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in hip-pocampal tissue were detected by qRT-PCR,the blood routine examination indicators(white blood cell,red blood cell and platelet counts)and liver and kidney function indicators(alanine aminotransferase,aspartate aminotransferase and creati-nine)were measured by routine methods.Results Compared with the wild-type group,the platform crossing times and per-centage of time spent in the target quadrant of mice in the 5×FAD transgenic were significantly decreased(all P<0.05),the microglia cells in the mice were obviously activated with increased neurite,the relative mRNA expressions of IL-1β,IL-6 and TNF-α in hippocampal tissue were significantly increased(all P<0.05).Compared with the 5×FAD transgenic group,the platform crossing times and percentage of time spent in the target quadrant of mice in the 5×FAD transgenic+APS group were significantly increased(all P<0.05),the quantity of activated microglia cells in the mice was obviously decreased,the relative mRNA expressions of IL-1β,IL-6 and TNF-α in hippocampal tissue were significantly decreased(all P<0.05).Conclusion Astragalus polysaccharides can improve cognitive function of 5×FAD transgenic mice via reg-ulating microglial activity and alleviating neuroinflammation.关键词
阿尔茨海默病/黄芪多糖/小胶质细胞/认知功能/神经炎症Key words
Alzheimer's disease/Astragalus polysaccharides/microglia/cognitive function/neuroinflammation分类
医药卫生引用本文复制引用
崔晓丽,魏振,林姿,陈子博,李超,杜思哲..黄芪多糖对阿尔茨海默病小鼠认知功能及小胶质细胞介导神经炎症的影响[J].现代中西医结合杂志,2025,34(15):2065-2069,2088,6.基金项目
福建省自然科学基金项目(2022J01849) （2022J01849）
福建省中青年教师教育科研项目(JAT210165) （JAT210165）
