中国临床药理学杂志2025，Vol.41Issue(16)：2287-2292,6.DOI:10.13699/j.cnki.1001-6821.2025.16.007
达托霉素对骨肉瘤U266细胞生长的影响
The effect of daptomycin on the growth of osteosarcoma U266 cells
摘要
Abstract
Objective To investigate the effects of daptomycin(DAP)on the proliferation,apoptosis,and cell cycle of U266[U266B1]human multiple myeloma cells(U266).Methods U266 cells were divided into the following groups:normal control group(NC group),DAP 20μM group(DAP20),DAP 40 μM group(DAP40),DAP 80 μM group(DAP80),BZ 50 nM group(BZ50),and DAP 80 μM+BZ 50 nM group(DAP80+BZ50).U266 cells were treated with varying concentrations of DAP(0,20,40,and 80 μM),50 nM bortezomib(BZ),and combination of DAP(80 μM)plus BZ(50 nM).Effects were assessed using cell counting kit-8(CCK-8)assays,Western blotting(WB),flow cytometry,and quantitative real-time polymerase chain reaction(qPCR).Results At 24 h post-treatment:Cell viability rates were recorded as(97.13±2.51)％,(96.80±3.44)％,(85.48±3.28)％,(81.56±2.09)％,(60.78±2.80)％,and(38.09±2.09)％for DAP alone(0-80 μM),BZ monotherapy,and combinatorial treatment,respectively.Early apoptotic cell proportions measured via flow cytometry showed values of(7.50±0.84)％,(8.20±1.41)％,(9.07±1.22)％,(13.14±2.27)％,(14.51±2.58)％,and(15.17±1.87)％across groups.Proportions of cells in G1 phase were determined to be(33.40±1.48)％,(33.03±2.49)％,(31.50±1.40)％,(38.59±1.54)％,(36.94±1.13)％,and(39.43±1.40)％.Relative expression levels of ribosomal protein S19(RPS19)mRNA exhibited fold changes of 0.99±0.09,1.00±0.14,0.66±0.04,0.61±0.06,0.55±0.04,and 0.53±0.07,while corresponding protein levels via WB analysis were 1.08±0.05,0.97±0.03,0.90±0.02,0.87±0.04,0.89±0.04,and 0.57±0.03.Statistically significant differences(all P＜0.001)were observed in BZ50,DAP80,and their combination compared to DAP 0 μM group.Conclusion DAP may exert its inhibitory effect on U266 cell proliferation and promote apoptosis by downregulating the expression of RPS19.This study provides a potential therapeutic drug for the treatment of multiple myeloma.关键词
达托霉素/骨髓瘤细胞/U266细胞/核糖体蛋白S19Key words
daptomycin/myeloma cells/U266 cells/RPS19分类
医药卫生引用本文复制引用
陈彩云,陈金成,许秋霞,庄奕筠..达托霉素对骨肉瘤U266细胞生长的影响[J].中国临床药理学杂志,2025,41(16):2287-2292,6.基金项目
福建省自然科学基金项目(2021J01253) （2021J01253）
