中国比较医学杂志2025，Vol.35Issue(9)：50-59,10.DOI:10.3969/j.issn.1671-7856.2025.09.005
基于生物信息学及体外实验分析PD-L1调控口腔癌转移机制研究
Mechanism of programmed death-ligand 1 in regulating oral cancer metastasis based on bioinformatics and in vitro experiments
摘要
Abstract
Objective To analyze the role and mechanism of PD-L1 in oral cancer metastasis based on TCGA and GEO databases.Methods The expression characteristics and clinical significance of the PD-L1 in oral cancer were analyzed using the TCGA database.PD-L1 mRNA levels were detected by quantitative reverse transcription-polymerase chain reaction(RT-qPCR)in various oral cancer cell lines.CCK-8,scrath test,Transwell-migration,and matrigel-invasion assays were employed to assess the effects of PD-L1 on proliferation,migration,and invasion of oral cancer cells.The interaction network between PD-L1 and functional genes in patients with oral cancer was constructed using STRING software and the GEO database,and key pathways were screened by Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.The regulatory relationship between PD-L1 and key genes was validated by RT-qPCR.Results TCGA data revealed that PD-L1 was highly expressed in patients with oral cancer and was correlated with lymph node metastasis(P＜0.01).PD-L1 was also highly expressed in oral cancer cell lines and its inhibition significantly inhibited the proliferation,migration,and invasion of Cal27 and SCC25 cells(P＜0.05).KEGG analysis indicated that PD-L1 activated the Janus kinase(JAK)/signal transducer and activator of transcription(STAT)pathway by upregulating C-X-C motif chemokine ligand(CXCL)9 and CXCL10,thereby promoting STAT1 expression to regulate oral cancer metastasis.Inhibition of the JAK/STAT pathway further suppressed the proliferation,migration,invasion,and expression of STAT1,CXCL9,and CXCL10 in Cal27 and SCC25 cells(P＜0.05).Conclusions PD-L1 may promote oral cancer cell proliferation,migration,and invasion by upregulating CXCL9 and CXCL10 to regulate the JAK/STAT pathway and enhance STAT1 expression,ultimately driving oral cancer growth and metastasis.关键词
生物信息学/口腔癌/PD-L1/细胞转移/JAK/STAT通路Key words
bioinformatics/oral cancer/PD-L1/cell metastasis/JAK/STAT pathway分类
医药卫生引用本文复制引用
王恬,聂晓翠,王晓堂,高继萍,宋晓娜,宋国华..基于生物信息学及体外实验分析PD-L1调控口腔癌转移机制研究[J].中国比较医学杂志,2025,35(9):50-59,10.基金项目
山西省中央引导地方科技发展资金项目(YDZJSX2022A060) （YDZJSX2022A060）
山西省科技创新人才团队项目(202204051002032) （202204051002032）
山西省高等教育"百亿工程"科技引导专项(BYJL016). （BYJL016）
