中国光学（中英文）2025，Vol.18Issue(5)：1055-1065,11.DOI:10.37188/CO.2025-0045
流动相单分子免疫检测系统的设计
Design of flow-phase single-molecule immunoassay detection system
摘要
Abstract
We develop a single-molecule immunoassay detection system for flow-phase samples to enable early diagnosis of tumor biomarkers.The system includes an optical fluorescence imaging platform and an image processing algorithm.First,we developed a flow-phase single-molecule immunoassay detection meth-od suitable for real-time detection of low-concentration,high-throughput samples.Second,we designed a set of fluorescence imaging detection platform compatible with microfluidic chips.Through the rational config-uration of optical filters and beam splitters,high-resolution fluorescence imaging was achieved by utilizing multi-module integration.Finally,based on the image detection and counting algorithm for out-of-focus particles,we optimized a feature-matching algorithm to effectively detect out-of-focus fluorescent particles.Experimental results demonstrate a detection limit of 0.001 pg/mL within a linear range of 0.001-1 pg/mL,with coefficient of variation below 10％.The system can process up to 10 samples per hour.These findings indicate that our system meets the requirements for stable,sensitive,and high-throughput single-molecule im-munoassay detection,showing promising potential for early cancer screening.关键词
早期诊断/单分子免疫检测/荧光成像/光学平台/特征匹配Key words
early diagnosis/single-molecule immunodetection/fluorescence imaging/optical platform/fea-ture matching分类
数理科学引用本文复制引用
胡鹏涛,高若谦,葛明锋,董文飞,李力,苏新然..流动相单分子免疫检测系统的设计[J].中国光学（中英文）,2025,18(5):1055-1065,11.基金项目
国家重点研发计划(No.2022YFC2403502) （No.2022YFC2403502）
吉林省与中国科学院科技合作高新技术产业化专项资金项目(No.2023SYHZ0037) （No.2023SYHZ0037）
山东省青年自然科学基金项目(No.ZR2023QF136)Supported by the National Key R&D Program of China(No.2022YFC2403502) （No.ZR2023QF136）
Science and Technology Co-operation Special Project,Jilin Province and Chinese Academy of Sciences(No.2023SYHZ0037) （No.2023SYHZ0037）
Shandong Provincial Natural Science Foundation,China(No.ZR2023QF136) （No.ZR2023QF136）
