中国中医急症2025，Vol.34Issue(9)：1441-1446,6.DOI:10.3969/j.issn.1004-745X.2025.09.001
重症心痹方调控焦亡关键蛋白NLRP3、ASC、caspase1干预肠源性脓毒症合并心肌损伤的作用机制研究
Study on the Mechanism of Zhongzheng Xinbi Formula in Intervening Myocardial Injury in Enterogenic Sepsis by Regulating Pyroptosis Key Proteins NLRP3,ASC,and Caspase1
摘要
Abstract
Objective:To investigate the protective effects of Zhongzheng Xinbi Formula(ZZXBF)on myocardi-al injury induced by enterogenic sepsis and to explore its pharmacological mechanisms.Methods:Rats were pre-treated with ZZXBF,and the blood components of ZZXBF were identified using liquid chromatography-tandem mass spectrometry(LC-MS/MS).Before modeling,the remaining rats were randomly divided into the sham opera-tion group(only abdominal incision and suture),the model group,the positive drug group(intraperitoneal injection of methylprednisolone 8 mg/kg),and the ZZXBF group(oral gavage of ZZXBF).A rat model of sepsis-induced myocardial injury was established using the cecal ligation and puncture(CLP)method.HE staining was used to ob-serve pathological changes in cardiac tissue;an automatic biochemical analyzer was employed to measure the lev-els of CK,CK-MB,and LDH;immunohistochemistry was performed to detect the expression levels of pyroptosis key proteins NLRP3,ASC,and Cleaved-caspase1 in cardiac tissue;Western blotting was used to assess the pro-tein levels of ASC and Cleaved-caspase1 in cardiac tissue.Results:A total of 164 blood components of ZZXBF were detected.Compared with the sham operation group,the model group exhibited significant pathological damage to myocardial tissue and a significantly increased pathological score(P＜0.05);levels of CK,CK-MB,and LDH were significantly increased(P＜0.05);the ratios of integrated optical density(IOD)to area(AREA)for NLRP3,ASC,and Cleaved-caspase1 proteins were significantly increased(P＜0.05);and the protein levels of ASC and Cleaved-caspase1 were significantly increased(P＜0.05).Compared with the model group,ZZXBF intervention sig-nificantly reduced the myocardial pathological injury score and the levels of CK,CK-MB,and LDH induced by en-terogenic sepsis(all P＜0.05),significantly decreased the IOD/AREA ratios of NLRP3,ASC,and Cleaved-cas-pase1 proteins(P＜0.001),suppressed the expression level of the pyroptosis key protein Cleaved-caspase1(P＜0.01),and downregulated the expression level of ASC protein(P＞0.05).Conclusion:ZZXBF effectively amelio-rates myocardial injury in enterogenic sepsis by inhibiting the expression of key pyroptosis proteins,thereby antago-nizing pyroptosis.关键词
肠源性脓毒症/心肌损伤/重症心痹方/细胞焦亡/大鼠Key words
Enterogenic sepsis/Myocardial injury/Zhongzheng Xinbi Formula/Pyroptosis/Rats分类
医药卫生引用本文复制引用
严静,谢娟娟,刘园园,谭金晶,王光耀,黎丽群,谢胜..重症心痹方调控焦亡关键蛋白NLRP3、ASC、caspase1干预肠源性脓毒症合并心肌损伤的作用机制研究[J].中国中医急症,2025,34(9):1441-1446,6.基金项目
国家自然科学基金青年科学基金项目(82305151) （82305151）
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023167) （zyyzdxk-2023167）
广西研究生教育创新计划项目(YCBZ2024153) （YCBZ2024153）
广西重点研发项目(桂科AB23026098) （桂科AB23026098）
广西中医药多学科交叉创新团队(GZKJ2307) （GZKJ2307）
广西中医药管理局桂派中医大师工作室(GZY2024004) （GZY2024004）
广西医疗卫生重点(培育)学科(桂卫科教发[2023]1号) （培育）
