中国中医急症2025，Vol.34Issue(9)：1447-1451,5.DOI:10.3969/j.issn.1004-745X.2025.09.002
参蛤益肺胶囊对脂多糖诱导急性肺损伤小鼠的miRNA-21和TLR4/NF-κB信号通路的影响
Effect of Shenge Yifei Capsules on miRNA-21 and TLR4/NF-κB Signaling Pathway in Mice with LPS-induced Acute Lung Injury
摘要
Abstract
Objective:To investigate the protective effect of Shenge Yifei Capsules(SGYFC)on lipopolysaccha-ride(LPS)-induced acute lung injury(ALI)in mice and to explore whether its mechanism involves the regulation of miRNA-21 expression and the TLR4/NF-κB signaling pathway.Methods:60 Balb/c mice were randomly divid-ed into six groups(n=10 per group):sham group,model group,dexamethasone group,and low,medium,and high-dose SGYFC groups.Mice in the model group received intratracheal instillation of LPS(1 mg/kg,10 μL),while those in the sham group received an equal volume of sterile saline.After modeling,mice in the SGYFC groups were administered SGYFC via oral gavage at doses of 0.1,0.2,and 0.4 g/kg,respectively,once daily for 7 consecu-tive days.Mice in the dexamethasone group were given dexamethasone(0.4 mg/kg)via oral gavage for 3 days prior to modeling.Blood,bronchoalveolar lavage fluid(BALF),and lung tissues were collected 12 hours after modeling.Histopathological changes in lung tissue were examined using HE staining.The lung wet-to-dry(W/D)weight ra-tio was measured.The expression level of miRNA-21 in lung tissue was detected by quantitative real-time PCR(qRT-PCR).Protein expression levels of Toll-like receptor 4(TLR4)and nuclear factor kappa B(NF-κB)were analyzed by Western blotting.The levels of tumor necrosis factor-alpha(TNF-α)and interleukin-6(IL-6)in plas-ma and BALF were measured using ELISA.Results:Compared with the model group,treatment with SGYFC sig-nificantly alleviated inflammatory cell infiltration in the alveoli,reduced the lung W/D ratio(P＜0.01),increased miRNA-21 expression(P＜0.01),decreased the expression of TLR4 and NF-κB(P＜0.01),and significantly low-ered the levels of TNF-α and IL-6 in both serum and BALF(P＜0.01).Conclusion:SGYFC can alleviate LPS-induced ALI in mice.This protective effect may be associated with the upregulation of miRNA-21 and the inhibi-tion of the TLR4/NF-κB signaling pathway and inflammatory cytokine expression.关键词
急性肺损伤/参蛤益肺胶囊/miRNA/Toll样受体4/小鼠Key words
Acute lung injury/Shenge Yifei Capsules/miRNA/Toll-like receptor 4/Mice分类
医药卫生引用本文复制引用
黄江,邓健,王耀辉,林宗耀,程然,黄得艳,张胜男,杨云芳..参蛤益肺胶囊对脂多糖诱导急性肺损伤小鼠的miRNA-21和TLR4/NF-κB信号通路的影响[J].中国中医急症,2025,34(9):1447-1451,5.基金项目
国家中医优势专科建设单位-临床药学(国中医药医政函[2024]90号) （国中医药医政函[2024]90号）
2023年全国中药特色技术传承人才培训项目(国中医药人教函[2023]96号) （国中医药人教函[2023]96号）
四川省中医药管理局2023年度中医药科研专项课题面上项目(2023MS180) （2023MS180）
