中国药业2025，Vol.34Issue(19)：50-56,7.DOI:10.3969/j.issn.1006-4931.2025.19.010
胃肠炎患者肠道菌群特征分析
Characteristics of Intestinal Microbiota in Patients with Gastroenteritis
摘要
Abstract
Objective To investigate the characteristic alterations of intestinal microbiota in patients with gastroenteritis,and to provide a reference for the development of treatment plans and new drugs for patients with gastroenteritis.Methods Fecal samples were collected from 47 patients with gastroenteritis(Case group)and 44 healthy subjects(Control group)in the Ninth People's Hospital of Chongqing from February to August 2024.16S rRNA high-throughput sequencing technology combined with bioinformatics analysis was used to systematically compare the structural characteristics and functional differences of the intestinal microbiota between the two groups.Results Compared with those in the control group,the Chao1 index(P=0.000 11)and Shannon index(P=0.021)in the case group significantly reduced.The results of difference analysis in gut microbiota composition showed that the main differences between the two groups were Proteobacteria,Actinobacteria,Enterobacteriaceae,Streptococcaceae,Blautia,and Escherichia-Shigella.The results of linear discriminant analysis(LDA)showed that the Blautia isa signature microorganism of the intestinal microbiota in patients with gastroenteritis.Functional analysis showed that protein synthesis and amino acid metabolism pathways significantly reduced in patients with gastroenteritis.The random forest model showed that the Roseburia played an important role in distinguishing populations between the two groups.Conclusion The diversity of intestinal microbiota in patients with gastroenteritis significantly reduced compared to healthy subjects,and the structure of intestinal microbiota changes.These differential microbiota can be taken as potential biomarkers for gastroenteritis,which can provide a reference for early diagnosis,clinical treatment strategy formulation,and new drug development of intestinal microbiota in patients with gastroenteritis.关键词
胃肠炎/肠道菌群/16S rRNA高通量测序技术/菌群多样性/益生菌Key words
gastroenteritis/intestinal microbiota/16S rRNA high-throughput sequencing/bacterial diversity/probiotics分类
医药卫生引用本文复制引用
吕田甜,路晓钦,高源泽,冉文豪,王佾..胃肠炎患者肠道菌群特征分析[J].中国药业,2025,34(19):50-56,7.基金项目
重庆市科卫联合中医药技术创新与应用发展项目[2020ZY023931]. （）
