作物杂志Issue(5)：102-112,11.DOI:10.16035/j.issn.1001-7283.2025.05.014
玉米亚油酸合成相关基因的挖掘及转录调控机制研究
Study on the Mining and Transcriptional Regulation Mechanism of Genes Related to Linoleic Acid Synthesis in Maize
摘要
Abstract
Linoleic acid,as an unsaturated fatty acid,is essential for human health.Transcriptome analysis was carried out using self-bred high linoleic acid maize inbred line YM-2 and common maize inbred line Chang 7-2 as materials to explore candidate genes and key pathways related to linoleic acid biosynthesis in maize.The results showed that a total of 6505 differentially expressed genes(DEGs)were identified in the two comparison groups,of which 3381 were up-regulated and 3124 were down-regulated.KEGG enrichment analysis showed that these DEGs were significantly enriched in metabolic pathways such as linoleic acid metabolism(Zma00591)and fatty acid elongation(Zma00062).Among them,linoleic acid 9S-lipoxygenase(EC:1.13.11.58),linoleic acid 13S-lipoxygenase(EC:1.13.11.12),3-ketoacyl-CoA synthase(EC:2.3.1.199)and 17β-estradiol 17-dehydrogenase/very long chain 3-oxoacyl-CoA reductase(EC:1.1.1.62/1.1.1.330)were down-regulated,and related candidate genes LOX1,LOX3,LOX6,LOX10,LOX12,TS1,LOC103642039,and LOC103646607 may be directly related to the synthesis of linoleic acid.In order to verify the accuracy of RNA-seq data,we selected four genes with opposite expression patterns in the two materials for qRT-PCR verification,and found that the trend of gene expression was consistent with the transcriptome sequencing results.This study explored the genes and metabolic pathways related to linoleic acid biosynthesis in maize,which provided a reference for further analysis of the molecular regulation mechanism of high linoleic acid in maize,and also laid a foundation for molecular breeding of high oleic acid crops.关键词
玉米/亚油酸/转录组测序/差异表达基因/代谢途径Key words
Maize/Linoleic acid/Transcriptome sequencing/Differentially expressed genes/Metabolic pathway引用本文复制引用
杨珂,赖上坤,金倩,王卫军,丁翊东,刘晓飞..玉米亚油酸合成相关基因的挖掘及转录调控机制研究[J].作物杂志,2025,(5):102-112,11.基金项目
宿迁市农业科技自主创新项目(SQCX202406) （SQCX202406）
宿迁市科技计划项目(K202435) （K202435）
