作物杂志Issue(5)：135-141,7.DOI:10.16035/j.issn.1001-7283.2025.05.018
水稻CYP450家族基因Os78A5的表达分析
Expression Analysis of the CYP450 Family Gene Os78A5 in Rice
摘要
Abstract
Cytochrome P450(CYP450)is widely present in various organisms and plays an essential role in plant secondary metabolism and stress response.A gene encoding the CYP450 protein,Os78A5(LOC_Os11g29720),was cloned from rice(Oryza sativa).The gene structure,evolution process and cis-regulatory elements of Os78A5 were analyzed by bioinformatics method,and the expression of Os78A5 in different tissues and response to different plant hormones and abiotic stresses were analyzed by quantitative real-time PCR(qRT-PCR).The results showed that coding region of Os78A5 was 1617 bp,encoding 538 amino acids.Os78A5 has closer evolutionary relationship with the homologous protein CYP78A5 of Setaria viridis and Sorghum bicolor.The promoter region of Os78A5 contained 48 hormone response elements,23 environmental stress response elements and 35 water response cis-regulatory elements.The expression of Os78A5 in rice leaves was significantly higher than that in other tissues,and it was induced by 6-benzylaminopurine,gibberellic acid,indole acetic acid,abscisic acid,high temperature and low temperature,suggesting that Os78A5 might be involved in abiotic stress response in rice.关键词
水稻/CYP450/非生物胁迫/植物激素/表达分析Key words
Rice/CYP450/Abiotic stress/Plant hormone/Expression analysis引用本文复制引用
植藓闳,纪子贤,许镇旺,谭恩,梁日深,马帅鹏,唐辉武..水稻CYP450家族基因Os78A5的表达分析[J].作物杂志,2025,(5):135-141,7.基金项目
广东省自然科学基金(2022A1515010798) （2022A1515010798）
清远市科技计划项目(2023KJJ005) （2023KJJ005）
仲恺农业工程学院仲华基因产业学院项目(ZHCY2023007) （ZHCY2023007）
海南省自然科学基金面上项目(320MS079) （320MS079）
