中山大学学报（医学科学版）2025，Vol.46Issue(5)：806-815,10.
雄性小鼠衰老过程中睾丸和附睾长链非编码RNA的动态变化
Dynamics Changes of Long Non-Coding RNA in the Testis and Epididymis During Male Mouse Aging
摘要
Abstract
[Objective]To investigate the dynamic expression profiles and potential regulatory mechanisms of long non-coding RNAs(lncRNAs)in male reproductive system aging.[Methods]A naturally aging C57BL/6 mouse model was used and 4 mice were selected each at 3,15,and 21 months of age.RNA was extracted from seven regions of the male reproductive tract(testis,efferent duct,initial segment of epididymis,caput epididymis,corpus epididymis,cauda epididymis,and vas deferens),followed by RNA sequencing and bioinformatics analysis.[Results]Region-specific dynamic expression profiles of lncRNAs were constructed in the testis,epididymis(efferent duct,initial segment,caput,corpus,and cauda),and vas deferens of male mice.Combined with gene functional enrichment analysis,the functional associations of lncRNAs were elucidated in reproductive system aging.The differentially expressed lncRNAs in the aging testis were primarily involved in hormone biosynthesis and extracellular matrix organization,while those in the initial segment of the epididymis were closely related to cell recognition and epithelial cell migration.A comprehensive lncRNA expression atlas associated with male reproductive aging was established.[Conclusion]LncRNAs may participate in male reproductive aging through the regulation of the reproductive microenvironment,which provides key molecular targets and a research foundation for understanding age-related fertility decline.关键词
长链非编码RNA/男性生殖道/生殖衰老/睾丸/附睾Key words
long non-coding RNAs/male reproductive tract/reproductive aging/testis/epididymis分类
生物科学引用本文复制引用
何泓乐,孙睿,管锦鸿,陈若扬,谢云,莫承强,沙显燊,黄燕平..雄性小鼠衰老过程中睾丸和附睾长链非编码RNA的动态变化[J].中山大学学报（医学科学版）,2025,46(5):806-815,10.基金项目
广东省自然科学基金(2024A1515010094 （）
2023A1515012290) （）
广东大学生科技创新培育专项资金(pdjh2023b0018) （pdjh2023b0018）
广东省泌尿系统疾病临床医学研究中心资助项目(2020B1111170006) （2020B1111170006）
