LncRNA SNHG14通过靶向miR-579-3p/SPARC轴影响肝细胞癌Huh7细胞的恶性生物学行为OA北大核心

Objective:To investigate the effects of long non-coding RNA(lncRNA)small nucleolar RNA host gene 14(SNHG14)on the malignant biological behavior of hepatocellular carcinoma(HCC)cells by targeting miR-579-3p/secreted protein acidic and rich in cysteine(SPARC)axis.Methods:Normal human hepatocytes(LO2)and HCC cells(Huh7,Hep3B,HepG2)were routinely cultured.Huh7 cells were randomly divided into control,sh-NC,sh-SNHG14,sh-SNHG14+anti-NC,and sh-SNHG14+anti-miR-579-3p groups.The mRNA expression levels of SNHG14,miR-579-3p,and SPARC in the above cells were detected by qPCR.Dual-luciferase reporter gene assay was applied to verify the regulatory relationship between SNHG14 and miR-579-3p,as well as between miR-579-3p and SPARC.The proliferation,migration,invasion,and apoptosis of Huh7 cells in each group were assessed using MTT,wound-healing,Transwell,and flow cytometry assays,respectively.WB was used to detect the protein levels of PCNA,E-cadherin,vimentin,and SPARC.Results:In HCC cells,SNHG14 and SPARC mRNA were upregulated,whereas miR-579-3p was downregulated(all P<0.05).There was a direct binding regulatory relationship between SNHG14 and miR-579-3p,as well as between miR-579-3p and SPARC mRNA(all P<0.05).Knockdown of SNHG14 significantly inhibited the proliferation,migration,invasion,and the expression of PCNA and vimentin,as well as the mRNA and protein expression of SPARC in Huh7 cells(all P<0.05),while promoting apoptosis,expression of miR-579-3p,and E-cadherin expression(all P<0.05).Inhibition of miR-579-3p partially reversed the effects of SNHG14 knockdown on Huh7 cells(all P<0.05).Conclusion:Knockdown of SNHG14 can inhibit the malignant biological behaviors of Huh7 cells and promote their apoptosis by targeting the miR-579-3p/SPARC axis.The SNHG14/miR-579-3p/SPARC axis may represent a potential therapeutic target for HCC.