中国现代中药2025，Vol.27Issue(9)：1675-1682,8.DOI:10.13313/j.issn.1673-4890.20250331005
土鳖虫基原动物地鳖的性别鉴定
Sex Identification of the Original Animal Eupolyphaga sinensis of Eupolyphaga Steleophaga
摘要
Abstract
Objective:To explore the sex identification method of the original animal Eupolyphaga sinensis of Eupolyphaga Steleophaga due to the issue of mixed use in medicinal products.This study aims to provide a reference for improving the quality control of these medicinal materials.Methods:Morphological observation,including macro-traits,micro-traits,and microscopic examination,was performed on male and female larvae and adults of E.sinensis.DNA barcodes-cytochrome oxidase Ⅰ(COI),cytochrome b gene(Cytb),18S rRNA,and 16S rRNA-were used for species identification and sequence comparison between male and female samples across multiple batches.Results:The complete morphological characteristics of male and female E.sinensis larvae and adults were delineated.When E.sinensis specimens were intact,male and female larvae could be distinguished by the number of abdominal transverse annuli and the size of the genital operculum,while adults exhibited clear dimorphism of external features such as the presence or absence of wings.In the identification by DNA barcoding,all samples were accurately identified as E.sinensis,while there was no differential sites in the barcode between male and female individuals.Conclusion:The sex of E.sinensis can be identified by conventional identification techniques when the individual morphology is intact.However,conventional DNA barcoding has limitations for sex identification.Therefore,it is necessary to further study and develop a molecular method for sex identification of E.sinensis.关键词
土鳖虫/地鳖/性别鉴定/传统鉴定/DNA条形码Key words
Eupolyphaga Steleophaga/Eupolyphaga sinensis Walker/sex identification/conventional identification/DNA barcoding分类
医药卫生引用本文复制引用
邹何元,梁莹莹,吴文如,江珮瑜,张雨薇,来慧丽..土鳖虫基原动物地鳖的性别鉴定[J].中国现代中药,2025,27(9):1675-1682,8.基金项目
国家中医药管理局全国老药工传承工作室建设项目(国中医药人教函[2024]255号) （国中医药人教函[2024]255号）
2024年广东食品药品职业学院科研项目(2024ZR27) （2024ZR27）
广州中医药大学大学生创新创业训练计划项目(202410572308) （202410572308）
